Modified inoculum for radiometric susceptibility testing of Mycobacterium tuberculosis

Charles E. Stager, James R. Davis, Mario N. Saccomani, Corpus O. Ortigoza, Reuben D. Wende, James W. Raleigh

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Two-hundred and fifteen isolates of Mycobacterium tuberculosis were evaluated with the BACTEC 460 radiometric method for susceptibility to isoniazid, rifampin, ethambutol, and streptomycin (SM); a revised protocol for inoculum preparation was used. Fresh clinical isolates were subcultured into 7H9 broth and then photometrically adjusted to the equivalent of a 0.5 McFarland standard, one-half the recommended inoculum density. This method produced an overall 98.3% correlation with a conventional agar method. The sensitivity of this procedure was good for all drugs tested except for the lowest concentration of SM (2 μg/ml). Specificity was excellent for all drugs tested. After repeat testing, only four discrepancies were found, yielding a 99.8% correlation between the two systems. The time required for susceptibility tests averaged 4.6 days. This method for inoculum preparation effectively minimized the number of susceptibility tests exceeding the threshold value before the fourth day of incubation. This allowed for definite trends of the growth index values to become established before interpretation of results.

Original languageEnglish (US)
Pages (from-to)243-247
Number of pages5
JournalCurrent Microbiology
Volume17
Issue number5
DOIs
StatePublished - Sep 1 1988

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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