Modification of the colorimetric assay for chloramphenicol in the presence of bilirubin

Edward Mason, Sheldon Kaplan, C. J. Baker, D. A. Ritter

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The authors recently found that bilirubin interferes with the colorimetric chloramphenicol assay. Levels of serum bilirubin alone (4 to 6 mg/dl) resulted in apparent concentrations of chloramphenicol which appear to be in the therapeutic range. Concentrations of serum bilirubin greater than 8 mg/dl resulted in levels of apparent chloramphenicol associated with toxicity (>50 μg/ml). Small amounts of activated charcoal added to the isoamyl acetate extraction step of the assay eliminated this interference by bilirubin.

Original languageEnglish (US)
Pages (from-to)544-546
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume15
Issue number4
DOIs
StatePublished - 1979

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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