Modification of T lymphocytes to express tumor antigens

Aaron E. Foster, Xiao Tong Song

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Human T cells can be genetically modified to express tumor-associated antigens (TAA) for the induction of tumor-specific immunity, suggesting that T cells may be alternative candidates of effective antigenpresenting cells (TAPC) and may be useful in vivo as cellular cancer vaccines. The effective induction of TAA-specific T cell immune responses requires activation of T cells by CD3/CD28 antibodies and the presence of proinflammatory cytokines such as interleukin-7 (IL-7) and interleukin-12 (IL-12). Here, we describe the technique of preparing activated human TAPC pulsed with TAA peptides for the induction of tumor antigen-specific T cell immunity in vitro.

Original languageEnglish (US)
Pages (from-to)169-176
Number of pages8
JournalMethods in Molecular Biology
Volume1139
DOIs
StatePublished - 2014

Keywords

  • Antigen-presenting cells
  • Immunotherapy
  • Peptide pulsing
  • T cell immunity
  • T lymphocyte
  • TAPC
  • Tumor-associated antigen

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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