Modeling key metabolic pathways of cancer cells using label-free fluorescence lifetime imaging

Linghao Hu, Alex J. Walsh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Glycolysis, glutaminolysis, and oxidative phosphorylation (OXPHOS) are the main cellular metabolic pathways used to generate energy. Reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavin adenine dinucleotide (FAD) are coenzymes in these metabolism pathways, and detection of their endogenous fluorescence lifetime offers a label-free and quantitative method to study redox state and model cellular metabolism. Many cancer cells depend on glycolysis instead of oxidative phosphorylation to produce energy even in an aerobic environment, which is known as the Warburg effect. Here, autofluorescence lifetime images of NADH and FAD were obtained from MCF-7 breast cancer cells using multiphoton fluorescence lifetime microscopy. Cells were cultured in the Dulbecco's Modified Eagle's Medium (DMEM), and glycolysis, glutaminolysis, and OXPHOS were inhibited using 2-DG (2-Dexoy-D-glucose), sodium cyanide, and BPTES (Bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl) ethyl sulfide) respectively. The fraction of free NADH decreases when glycolysis is inhibited and increases when OXPHOS is inhibited. The mean NADH lifetime is increased when glycolysis is inhibited and is reduced when OXPHOS is inhibited. NADH and FAD fluorescence lifetime features vary when inhibiting glutaminolysis. Altogether, this investigation offers a non-invasive method to image key metabolic pathways at a cellular level, which improves the identification of different metabolic states and drug responses of cancer cells.

Original languageEnglish (US)
Title of host publicationLabel-free Biomedical Imaging and Sensing (LBIS) 2022
EditorsNatan T. Shaked, Oliver Hayden
PublisherSPIE
ISBN (Electronic)9781510648159
DOIs
StatePublished - 2022
EventLabel-free Biomedical Imaging and Sensing (LBIS) 2022 - Virtual, Online
Duration: Feb 20 2022Feb 24 2022

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11972
ISSN (Print)1605-7422

Conference

ConferenceLabel-free Biomedical Imaging and Sensing (LBIS) 2022
CityVirtual, Online
Period2/20/222/24/22

Keywords

  • cancer cell
  • FAD
  • fluorescence lifetime
  • label-free
  • metabolism
  • NADH

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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