Mobilization of CD34+ progenitor cells by granulocyte colony- stimulating factor in human immunodeficiency virus type 1-infected adults

Karen S. Slobod, Traci A. Bennett, Pamela J. Freiden, Amer M. Kechli, Nanna Howlett, Patricia M. Flynn, David R. Head, Deo K. Srivastava, James M. Boyett, Malcolm K. Brenner, J. Victor Garcia

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

We conducted a clinical trial to determine the feasibility of growth factor mobilization of CD34+ progenitor cells in human immunodeficiency virus type 1 (HIV-1)-infected individuals. Eight asymptomatic, HIV-l-infected adults (median CD4+ T-cell count, 415 cellsμL), received 480 μg/d of granulocyte colony-stimulating factor (G-CSF) for 6 days without evidence of vital activation. Despite concerns that HIV-1 might inhibit hematopoiesis, CD34+ cells were successfully mobilized to the periphery of all donors, independent of the baseline CD4+ T-cell count, and the status of antiretroviral therapy. Leukapheresis was performed on day 6, and yielded a median of 194 x 106 CD34+ cells per leukapheresis (n = 7). CD34-enriched cells from the leukapheresia were predominantly myeloid-committed, but between 0.2% and 1.7% were primitive CD34+/CD38- progenitors. A median of 21.7% of the mobilized CD34+ cells were dimly positive for CD4. Consequently, CD34+-enriched cells were purified on the call sorter (mean purity, 97.7% ± 2.4%; n = 7), and examined for HIV-1 DNA. Purified CD34+ cells from two of seven donors were polymerase chain reaction (PCR)-positive for HIV-1, but only from one of three samples from each donor. We conclude that G-CSF can safely mobilize CD34+ progenitor cells in HIV-l-infected subjects, and that these cells are suitable for consideration in gene- transfer strategies.

Original languageEnglish (US)
Pages (from-to)3329-3335
Number of pages7
JournalBlood
Volume88
Issue number9
DOIs
StatePublished - Nov 1 1996

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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