miR-29 regulates Tet1 expression and contributes to early differentiation of mouse ESCs

Yanhua Cui, Ting Li, Dehua Yang, Song Li, Weidong Le

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The ten-eleven translocation-1 (Tet1), which converts 5-methylcytosine (5mC) to 5-hydroxymethycytosine (5hmC), plays important roles in many important biological processes, such as mouse embryonic stem cells (ESCs) maintenance. However, the mechanisms for Tet-1 regulation remain largely unknown. Here we showed that miR-29 family (miR-29a, miR-29b and miR-29c) can directly repress Tet1 expression. We found that Tet1 was highly expressed and 5hmC was presented at relatively high levels in mouse ESCs, but the levels of both Tet1 and 5hmC were reduced during the early differentiation of ESCs. On the contrary, miR-29 level was increased in this process. ESCs stably transfecting with miR-29 precursors showed lower levels of Tet1 protein and 5hmC. Furthermore, we demonstrated that miR-29 overexpression selectively affected cell lineage markers and skewed ESC differentiation, which was similar in Tet1 knockdown ESCs. Our results indicate that miR-29 is a direct regulator of Tet1 in mouse ESCs.

Original languageEnglish (US)
Pages (from-to)64932-64941
Number of pages10
JournalOncotarget
Volume7
Issue number40
DOIs
StatePublished - 2016

Keywords

  • 5hmC
  • Embryonic stem cells
  • MiR-29 family
  • Tet1

ASJC Scopus subject areas

  • Oncology

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