Microfluidic integration of nanomechanical resonators for protein analysis in serum

P. S. Waggoner, C. P. Tan, H. G. Craighead

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


We describe a technique for reversibly encapsulating nanomechanical resonant sensors within microfluidic channels that are reusable and easy to fabricate. We demonstrate the feasibility and sensitivity of our microfluidic system by detecting prostate specific antigen (PSA) at 1-100 fM concentrations in serum, using a nanoparticle-based mass-labeling sandwich assay. By comparing these results to our previous data for PSA detection, we observe enhanced assay sensitivity in a significantly reduced time with the microfluidic assay. In addition, we show that a binary mixture of the proteins vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) can be detected in serum using a single microfluidic channel. This work lays the groundwork for future applications of these nanomechanical resonant devices for highly sensitive, multiplexed protein analysis using microfluidics.

Original languageEnglish (US)
Pages (from-to)550-555
Number of pages6
JournalSensors and Actuators, B: Chemical
Issue number2
StatePublished - Oct 28 2010


  • Biosensor
  • Cantilever
  • MEMS
  • Microfluidic encapsulation
  • NEMS
  • Sandwich assay

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering


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