Microfabricated cantilevers for measurement of subcellular and molecular forces

Mark E. Fauver; Dwayne L. Dunaway; David H. Lilienfeld; Harold G. Craighead; Gerald H. Pollack

doi:10.1109/10.686797

Microfabricated cantilevers for measurement of subcellular and molecular forces

Mark E. Fauver, Dwayne L. Dunaway, David H. Lilienfeld, Harold G. Craighead, Gerald H. Pollack

Houston Methodist

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

We present two new microfabricated cantilever-beam force transducers. The transducers were fabricated from thin silicon-nitride films, and were used respectively to measure forces generated by two small-muscle preparations: the single myofibril, and the single actin filament in contact with a myosin-coated surface. A simple resonance method was developed to characterize the transducers. Because of the high reproducibility of lever dimensions and the consistency of the modulus of elasticity, few calibration measurements sufficed to characterize the stiffness of all the levers on a single wafer.

Original language	English (US)
Pages (from-to)	891-898
Number of pages	8
Journal	IEEE Transactions on Biomedical Engineering
Volume	45
Issue number	7
DOIs	https://doi.org/10.1109/10.686797
State	Published - Jul 1998

Keywords

Actin filament
Calibration
Force transducer
Myofibril
Nanofabrication

ASJC Scopus subject areas

Biomedical Engineering

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10.1109/10.686797

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title = "Microfabricated cantilevers for measurement of subcellular and molecular forces",

abstract = "We present two new microfabricated cantilever-beam force transducers. The transducers were fabricated from thin silicon-nitride films, and were used respectively to measure forces generated by two small-muscle preparations: the single myofibril, and the single actin filament in contact with a myosin-coated surface. A simple resonance method was developed to characterize the transducers. Because of the high reproducibility of lever dimensions and the consistency of the modulus of elasticity, few calibration measurements sufficed to characterize the stiffness of all the levers on a single wafer.",

keywords = "Actin filament, Calibration, Force transducer, Myofibril, Nanofabrication",

author = "Fauver, \{Mark E.\} and Dunaway, \{Dwayne L.\} and Lilienfeld, \{David H.\} and Craighead, \{Harold G.\} and Pollack, \{Gerald H.\}",

note = "Funding Information: Manuscript received May 28, 1997; revised November 21, 1997. This work was supported by the National Institutes of Health (NIH) under Grant HL 5 R01HL18676. Asterisk indicates corresponding author. *M. E. Fauver is with the Department of Bioengineering, University of Washington, Seattle, WA 98195-7962 USA (e-mail: [email protected]). D. L. Dunaway and G. H. Pollack are with the Department of Bioengineering, University of Washington, Seattle, WA 98195-7962 USA. D. H. Lilienfeld and H. G. Craighead are with Cornell Nanofabrication Facility, Knight Lab, Ithaca, NY 14853-5403 USA. Publisher Item Identifier S 0018-9294(98)04383-3. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1998",

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doi = "10.1109/10.686797",

language = "English (US)",

volume = "45",

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AU - Fauver, Mark E.

AU - Dunaway, Dwayne L.

AU - Lilienfeld, David H.

AU - Craighead, Harold G.

AU - Pollack, Gerald H.

N1 - Funding Information: Manuscript received May 28, 1997; revised November 21, 1997. This work was supported by the National Institutes of Health (NIH) under Grant HL 5 R01HL18676. Asterisk indicates corresponding author. *M. E. Fauver is with the Department of Bioengineering, University of Washington, Seattle, WA 98195-7962 USA (e-mail: [email protected]). D. L. Dunaway and G. H. Pollack are with the Department of Bioengineering, University of Washington, Seattle, WA 98195-7962 USA. D. H. Lilienfeld and H. G. Craighead are with Cornell Nanofabrication Facility, Knight Lab, Ithaca, NY 14853-5403 USA. Publisher Item Identifier S 0018-9294(98)04383-3. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1998/7

Y1 - 1998/7

N2 - We present two new microfabricated cantilever-beam force transducers. The transducers were fabricated from thin silicon-nitride films, and were used respectively to measure forces generated by two small-muscle preparations: the single myofibril, and the single actin filament in contact with a myosin-coated surface. A simple resonance method was developed to characterize the transducers. Because of the high reproducibility of lever dimensions and the consistency of the modulus of elasticity, few calibration measurements sufficed to characterize the stiffness of all the levers on a single wafer.

AB - We present two new microfabricated cantilever-beam force transducers. The transducers were fabricated from thin silicon-nitride films, and were used respectively to measure forces generated by two small-muscle preparations: the single myofibril, and the single actin filament in contact with a myosin-coated surface. A simple resonance method was developed to characterize the transducers. Because of the high reproducibility of lever dimensions and the consistency of the modulus of elasticity, few calibration measurements sufficed to characterize the stiffness of all the levers on a single wafer.

KW - Actin filament

KW - Calibration

KW - Force transducer

KW - Myofibril

KW - Nanofabrication

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JO - IEEE Transactions on Biomedical Engineering

JF - IEEE Transactions on Biomedical Engineering

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ER -

Microfabricated cantilevers for measurement of subcellular and molecular forces

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Keywords

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