Microarray transcription analysis of clinical Staphylococcus aureus isolates resistant to vancomycin

Emmanuel Mongodin, Jon Finan, Michael W. Climo, Adriana Rosato, Steven Gill, Gordon L. Archer

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

The transcriptomes of vancomycin intermediate-resistance Staphylococcus aureus (VISA) clinical isolates HIP5827 and Mu50 (MIC = 8 μg/ml) were compared to those of highly vancomycin-resistant S. aureus (VRSA; MIC = 32 μg/ml) passage derivatives by microarray. There were 35 genes with increased transcription and 16 genes with decreased transcription in common between the two VRSAs compared to those of their VISA parents. Of the 35 genes with increased transcription, 15 involved purine biosynthesis or transport, and the regulator (purR) of the major purine biosynthetic operon (purE-purD) was mutant. We hypothesize that increased energy (ATP) is required to generate the thicker cell walls that characterize resistant mutants.

Original languageEnglish (US)
Pages (from-to)4638-4643
Number of pages6
JournalJournal of bacteriology
Volume185
Issue number15
DOIs
StatePublished - Aug 2003

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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