Microarray transcription analysis of clinical Staphylococcus aureus isolates resistant to vancomycin

Emmanuel Mongodin; Jon Finan; Michael W. Climo; Adriana Rosato; Steven Gill; Gordon L. Archer

doi:10.1128/JB.185.15.4638-4643.2003

Microarray transcription analysis of clinical Staphylococcus aureus isolates resistant to vancomycin

Emmanuel Mongodin, Jon Finan, Michael W. Climo, Adriana Rosato, Steven Gill, Gordon L. Archer

Academic Institute

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

The transcriptomes of vancomycin intermediate-resistance Staphylococcus aureus (VISA) clinical isolates HIP5827 and Mu50 (MIC = 8 μg/ml) were compared to those of highly vancomycin-resistant S. aureus (VRSA; MIC = 32 μg/ml) passage derivatives by microarray. There were 35 genes with increased transcription and 16 genes with decreased transcription in common between the two VRSAs compared to those of their VISA parents. Of the 35 genes with increased transcription, 15 involved purine biosynthesis or transport, and the regulator (purR) of the major purine biosynthetic operon (purE-purD) was mutant. We hypothesize that increased energy (ATP) is required to generate the thicker cell walls that characterize resistant mutants.

Original language	English (US)
Pages (from-to)	4638-4643
Number of pages	6
Journal	Journal of bacteriology
Volume	185
Issue number	15
DOIs	https://doi.org/10.1128/JB.185.15.4638-4643.2003
State	Published - Aug 2003

ASJC Scopus subject areas

Microbiology
Molecular Biology

Access to Document

10.1128/JB.185.15.4638-4643.2003

Cite this

@article{83ac4277df374b8e98ab235482c0aa9c,

title = "Microarray transcription analysis of clinical Staphylococcus aureus isolates resistant to vancomycin",

abstract = "The transcriptomes of vancomycin intermediate-resistance Staphylococcus aureus (VISA) clinical isolates HIP5827 and Mu50 (MIC = 8 μg/ml) were compared to those of highly vancomycin-resistant S. aureus (VRSA; MIC = 32 μg/ml) passage derivatives by microarray. There were 35 genes with increased transcription and 16 genes with decreased transcription in common between the two VRSAs compared to those of their VISA parents. Of the 35 genes with increased transcription, 15 involved purine biosynthesis or transport, and the regulator (purR) of the major purine biosynthetic operon (purE-purD) was mutant. We hypothesize that increased energy (ATP) is required to generate the thicker cell walls that characterize resistant mutants.",

author = "Emmanuel Mongodin and Jon Finan and Climo, {Michael W.} and Adriana Rosato and Steven Gill and Archer, {Gordon L.}",

year = "2003",

month = aug,

doi = "10.1128/JB.185.15.4638-4643.2003",

language = "English (US)",

volume = "185",

pages = "4638--4643",

journal = "Journal of bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "15",

}

TY - JOUR

T1 - Microarray transcription analysis of clinical Staphylococcus aureus isolates resistant to vancomycin

AU - Mongodin, Emmanuel

AU - Finan, Jon

AU - Climo, Michael W.

AU - Rosato, Adriana

AU - Gill, Steven

AU - Archer, Gordon L.

PY - 2003/8

Y1 - 2003/8

N2 - The transcriptomes of vancomycin intermediate-resistance Staphylococcus aureus (VISA) clinical isolates HIP5827 and Mu50 (MIC = 8 μg/ml) were compared to those of highly vancomycin-resistant S. aureus (VRSA; MIC = 32 μg/ml) passage derivatives by microarray. There were 35 genes with increased transcription and 16 genes with decreased transcription in common between the two VRSAs compared to those of their VISA parents. Of the 35 genes with increased transcription, 15 involved purine biosynthesis or transport, and the regulator (purR) of the major purine biosynthetic operon (purE-purD) was mutant. We hypothesize that increased energy (ATP) is required to generate the thicker cell walls that characterize resistant mutants.

AB - The transcriptomes of vancomycin intermediate-resistance Staphylococcus aureus (VISA) clinical isolates HIP5827 and Mu50 (MIC = 8 μg/ml) were compared to those of highly vancomycin-resistant S. aureus (VRSA; MIC = 32 μg/ml) passage derivatives by microarray. There were 35 genes with increased transcription and 16 genes with decreased transcription in common between the two VRSAs compared to those of their VISA parents. Of the 35 genes with increased transcription, 15 involved purine biosynthesis or transport, and the regulator (purR) of the major purine biosynthetic operon (purE-purD) was mutant. We hypothesize that increased energy (ATP) is required to generate the thicker cell walls that characterize resistant mutants.

UR - http://www.scopus.com/inward/record.url?scp=0038781384&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038781384&partnerID=8YFLogxK

U2 - 10.1128/JB.185.15.4638-4643.2003

DO - 10.1128/JB.185.15.4638-4643.2003

M3 - Article

C2 - 12867477

AN - SCOPUS:0038781384

SN - 0021-9193

VL - 185

SP - 4638

EP - 4643

JO - Journal of bacteriology

JF - Journal of bacteriology

IS - 15

ER -

Microarray transcription analysis of clinical Staphylococcus aureus isolates resistant to vancomycin

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this