Micro and nanopatterned aminosilanes for covalent grafting of biomolecules in multiplexed microfluidic bioassays

S. Sathish; S. G. Ricoult; K. Toda-Peters; Amy Q. Shen

Micro and nanopatterned aminosilanes for covalent grafting of biomolecules in multiplexed microfluidic bioassays

S. Sathish, S. G. Ricoult, K. Toda-Peters, Amy Q. Shen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

With the growing demand for the miniaturization of biosensors, there is a need for new technologies to achieve efficient surface patterning of biomolecules at the micro- and nanoscale. Since most surface patterning approaches rely on the physisorption of biomolecules, the resulting bond is insufficient to withstand high shear stresses present in micro/nano fluidic devices. Here, we developed a microcontact printing (μCP) approach that enables micro- and nanopatterning of (3-aminopropyl)triethoxysilane (APTES) in a microfluidic device, to covalently graft multiple biomolecules such as proteins and DNA aptamers onto the patterned sites of the device substrate and demonstrated the capability of this integrated platform for immunoassays.

Original language	English (US)
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	1148-1149
Number of pages	2
ISBN (Electronic)	9780979806490
State	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: Oct 9 2016 → Oct 13 2016

Other

Other	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	10/9/16 → 10/13/16

Keywords

Aminosilanes
Micro/nanopatterns
Microcontact printing
Microfluidic biosensors

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

Micro and nanopatterned aminosilanes for covalent grafting of biomolecules in multiplexed microfluidic bioassays. / Sathish, S.; Ricoult, S. G.; Toda-Peters, K. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 1148-1149.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Sathish, S, Ricoult, SG, Toda-Peters, K & Shen, AQ 2016, Micro and nanopatterned aminosilanes for covalent grafting of biomolecules in multiplexed microfluidic bioassays. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, pp. 1148-1149, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 10/9/16.

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abstract = "With the growing demand for the miniaturization of biosensors, there is a need for new technologies to achieve efficient surface patterning of biomolecules at the micro- and nanoscale. Since most surface patterning approaches rely on the physisorption of biomolecules, the resulting bond is insufficient to withstand high shear stresses present in micro/nano fluidic devices. Here, we developed a microcontact printing (μCP) approach that enables micro- and nanopatterning of (3-aminopropyl)triethoxysilane (APTES) in a microfluidic device, to covalently graft multiple biomolecules such as proteins and DNA aptamers onto the patterned sites of the device substrate and demonstrated the capability of this integrated platform for immunoassays.",

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AU - Sathish, S.

AU - Ricoult, S. G.

AU - Toda-Peters, K.

AU - Shen, Amy Q.

PY - 2016

Y1 - 2016

N2 - With the growing demand for the miniaturization of biosensors, there is a need for new technologies to achieve efficient surface patterning of biomolecules at the micro- and nanoscale. Since most surface patterning approaches rely on the physisorption of biomolecules, the resulting bond is insufficient to withstand high shear stresses present in micro/nano fluidic devices. Here, we developed a microcontact printing (μCP) approach that enables micro- and nanopatterning of (3-aminopropyl)triethoxysilane (APTES) in a microfluidic device, to covalently graft multiple biomolecules such as proteins and DNA aptamers onto the patterned sites of the device substrate and demonstrated the capability of this integrated platform for immunoassays.

AB - With the growing demand for the miniaturization of biosensors, there is a need for new technologies to achieve efficient surface patterning of biomolecules at the micro- and nanoscale. Since most surface patterning approaches rely on the physisorption of biomolecules, the resulting bond is insufficient to withstand high shear stresses present in micro/nano fluidic devices. Here, we developed a microcontact printing (μCP) approach that enables micro- and nanopatterning of (3-aminopropyl)triethoxysilane (APTES) in a microfluidic device, to covalently graft multiple biomolecules such as proteins and DNA aptamers onto the patterned sites of the device substrate and demonstrated the capability of this integrated platform for immunoassays.

KW - Aminosilanes

KW - Micro/nanopatterns

KW - Microcontact printing

KW - Microfluidic biosensors

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ER -

Micro and nanopatterned aminosilanes for covalent grafting of biomolecules in multiplexed microfluidic bioassays

Abstract

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Keywords

ASJC Scopus subject areas

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