Micro and nanopatterned aminosilanes for covalent grafting of biomolecules in multiplexed microfluidic bioassays

S. Sathish, S. G. Ricoult, K. Toda-Peters, Amy Q. Shen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

With the growing demand for the miniaturization of biosensors, there is a need for new technologies to achieve efficient surface patterning of biomolecules at the micro- and nanoscale. Since most surface patterning approaches rely on the physisorption of biomolecules, the resulting bond is insufficient to withstand high shear stresses present in micro/nano fluidic devices. Here, we developed a microcontact printing (μCP) approach that enables micro- and nanopatterning of (3-aminopropyl)triethoxysilane (APTES) in a microfluidic device, to covalently graft multiple biomolecules such as proteins and DNA aptamers onto the patterned sites of the device substrate and demonstrated the capability of this integrated platform for immunoassays.

Original languageEnglish (US)
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages1148-1149
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: Oct 9 2016Oct 13 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period10/9/1610/13/16

Keywords

  • Aminosilanes
  • Micro/nanopatterns
  • Microcontact printing
  • Microfluidic biosensors

ASJC Scopus subject areas

  • Control and Systems Engineering

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