Metallofullerol nanoparticles with low toxicity inhibit tumor growth by induction of G0/G1 arrest

Jie Meng, Jianmin Xing, Xiaowei Ma, Weipeng Cao, Juan Lu, Yingze Wang, Xueyun Gao, Baoyun Sun, Xingjie Liang, Yuliang Zhao

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Aims: [Gd@C82(OH)22]n is a new type of nanoparticle with potent antineoplastic activity and low toxicity compared with traditional drugs. In this study, we explored, for the first time, the effect of [Gd@C82(OH)22]n on the cell cycle using human breast cancer MCF-7 and human umbilical vein endothelial ECV304 cell lines by flow cytometry. Methods: Cell viability was assessed through CCK-8 assay, and MCF-7 tumor-bearing mice were examined after 2 weeks of treatment with [Gd@C82(OH)22]n. Cell cycle-related gene expression was detected by microarray and confirmed by real-time PCR and RNAi. Results: Cell viability studies confirmed that [Gd@C82(OH) 22]n inhibits breast cancer effectively with very low toxicity. Flow cytometric data and microarray results reveal that [Gd@C 82(OH)22]n mediates G0/G1 arrest in both cell lines by regulating the expression of several genes, such as cyclin D2, cyclin E and CDK4, among others, in the related cell cycle. Conclusion: Results further demonstrated that [Gd@C82(OH)22]n could inhibit tumor growth by inducing tumor cell and vein endothelial cell G0/G1 arrest, which may explain the low toxicity of [Gd@C82(OH)22] n.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalNanomedicine
Volume8
Issue number2
DOIs
StatePublished - Feb 2013

Keywords

  • cell cycle
  • G0/G1 arrest
  • metallofullerene
  • nanomedicine
  • nanoparticle

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

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