Metallofullerene nanoparticles promote osteogenic differentiation of bone marrow stromal cells through BMP signaling pathway

Kangning Yang; Weipeng Cao; Xiaohong Hao; Xue Xue; Jing Zhao; Juan Liu; Yuliang Zhao; Jie Meng; Baoyun Sun; Jinchao Zhang; Xing Jie Liang

doi:10.1039/c2nr33575a

Metallofullerene nanoparticles promote osteogenic differentiation of bone marrow stromal cells through BMP signaling pathway

Kangning Yang, Weipeng Cao, Xiaohong Hao, Xue Xue, Jing Zhao, Juan Liu, Yuliang Zhao, Jie Meng, Baoyun Sun, Jinchao Zhang, Xing Jie Liang

Houston Methodist

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

Although endohedral metallofullerenol [Gd@C₈₂(OH) ₂₂]_n nanoparticles have anti-tumor efficiency and mostly deposit in the bones of mice, how these nanoparticles act in bone marrow stromal cells (MSCs) remains largely unknown. Herein, we observed that [Gd@C ₈₂(OH)₂₂]_n nanoparticles facilitated the differentiation of MSCs toward osteoblasts, as evidenced by the enhancement of alkaline phosphatase (ALP) activity and mineralized nodule formation upon [Gd@C₈₂(OH)₂₂]_n nanoparticle treatment. Mechanistically, the effect of [Gd@C₈₂(OH)₂₂]_n nanoparticles on ALP activity was inhibited by the addition of noggin as an inhibitor of the BMP signaling pathway. Moreover, the in vivo results of the ovariectomized rats further indicated that [Gd@C₈₂(OH) ₂₂]_n nanoparticles effectively improved bone density and prevented osteoporosis.

Original language	English (US)
Pages (from-to)	1205-1212
Number of pages	8
Journal	Nanoscale
Volume	5
Issue number	3
DOIs	https://doi.org/10.1039/c2nr33575a
State	Published - Feb 7 2013

ASJC Scopus subject areas

General Materials Science

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title = "Metallofullerene nanoparticles promote osteogenic differentiation of bone marrow stromal cells through BMP signaling pathway",

abstract = "Although endohedral metallofullerenol [Gd@C82(OH) 22]n nanoparticles have anti-tumor efficiency and mostly deposit in the bones of mice, how these nanoparticles act in bone marrow stromal cells (MSCs) remains largely unknown. Herein, we observed that [Gd@C 82(OH)22]n nanoparticles facilitated the differentiation of MSCs toward osteoblasts, as evidenced by the enhancement of alkaline phosphatase (ALP) activity and mineralized nodule formation upon [Gd@C82(OH)22]n nanoparticle treatment. Mechanistically, the effect of [Gd@C82(OH)22]n nanoparticles on ALP activity was inhibited by the addition of noggin as an inhibitor of the BMP signaling pathway. Moreover, the in vivo results of the ovariectomized rats further indicated that [Gd@C82(OH) 22]n nanoparticles effectively improved bone density and prevented osteoporosis.",

author = "Kangning Yang and Weipeng Cao and Xiaohong Hao and Xue Xue and Jing Zhao and Juan Liu and Yuliang Zhao and Jie Meng and Baoyun Sun and Jinchao Zhang and Liang, {Xing Jie}",

year = "2013",

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doi = "10.1039/c2nr33575a",

language = "English (US)",

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TY - JOUR

T1 - Metallofullerene nanoparticles promote osteogenic differentiation of bone marrow stromal cells through BMP signaling pathway

AU - Yang, Kangning

AU - Cao, Weipeng

AU - Hao, Xiaohong

AU - Xue, Xue

AU - Zhao, Jing

AU - Liu, Juan

AU - Zhao, Yuliang

AU - Meng, Jie

AU - Sun, Baoyun

AU - Zhang, Jinchao

AU - Liang, Xing Jie

PY - 2013/2/7

Y1 - 2013/2/7

N2 - Although endohedral metallofullerenol [Gd@C82(OH) 22]n nanoparticles have anti-tumor efficiency and mostly deposit in the bones of mice, how these nanoparticles act in bone marrow stromal cells (MSCs) remains largely unknown. Herein, we observed that [Gd@C 82(OH)22]n nanoparticles facilitated the differentiation of MSCs toward osteoblasts, as evidenced by the enhancement of alkaline phosphatase (ALP) activity and mineralized nodule formation upon [Gd@C82(OH)22]n nanoparticle treatment. Mechanistically, the effect of [Gd@C82(OH)22]n nanoparticles on ALP activity was inhibited by the addition of noggin as an inhibitor of the BMP signaling pathway. Moreover, the in vivo results of the ovariectomized rats further indicated that [Gd@C82(OH) 22]n nanoparticles effectively improved bone density and prevented osteoporosis.

AB - Although endohedral metallofullerenol [Gd@C82(OH) 22]n nanoparticles have anti-tumor efficiency and mostly deposit in the bones of mice, how these nanoparticles act in bone marrow stromal cells (MSCs) remains largely unknown. Herein, we observed that [Gd@C 82(OH)22]n nanoparticles facilitated the differentiation of MSCs toward osteoblasts, as evidenced by the enhancement of alkaline phosphatase (ALP) activity and mineralized nodule formation upon [Gd@C82(OH)22]n nanoparticle treatment. Mechanistically, the effect of [Gd@C82(OH)22]n nanoparticles on ALP activity was inhibited by the addition of noggin as an inhibitor of the BMP signaling pathway. Moreover, the in vivo results of the ovariectomized rats further indicated that [Gd@C82(OH) 22]n nanoparticles effectively improved bone density and prevented osteoporosis.

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DO - 10.1039/c2nr33575a

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EP - 1212

JO - Nanoscale

JF - Nanoscale

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ER -

Metallofullerene nanoparticles promote osteogenic differentiation of bone marrow stromal cells through BMP signaling pathway

Abstract

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