Abstract

Earlier studies on glucose metabolism in B-cells suggested an active TCA cycle in both naïve B cells and differentiated IgA plasma cells. Glycolysis was shown to be more active in IgA plasma cells than naïve B-cells. There have been no reports on the metabolism of fructose in B-cells. Fructose is a major sugar present in the western diet. Thus, we have investigated the metabolism of fructose in B-cells including the effect of glucose on the metabolism of fructose. In this study, using 13C NMR spectroscopy and [U-13C]fructose and [U-13C]glucose as stable 13C isotope tracers, we investigated the metabolic fate of fructose and glucose in B-cells. B-cells showed mitochondrial oxidation of fructose when administered alone, but showed diminished oxidation of fructose in the presence of glucose. On the other hand, fructose did not significantly affect the mitochondrial metabolism of glucose.

Original languageEnglish (US)
Pages (from-to)110-117
Number of pages8
JournalAnalytical Biochemistry
Volume552
DOIs
StatePublished - Jul 1 2018

Keywords

  • C isotope tracers
  • B-cells
  • Fructose metabolism
  • Mitochondrial oxidation
  • NMR spectroscopy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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