@article{c6cea50ce88b46c1bd806d1a7a183396,
title = "Metabolism of fructose in B-cells: A 13C NMR spectroscopy based stable isotope tracer study",
abstract = "Earlier studies on glucose metabolism in B-cells suggested an active TCA cycle in both na{\"i}ve B cells and differentiated IgA plasma cells. Glycolysis was shown to be more active in IgA plasma cells than na{\"i}ve B-cells. There have been no reports on the metabolism of fructose in B-cells. Fructose is a major sugar present in the western diet. Thus, we have investigated the metabolism of fructose in B-cells including the effect of glucose on the metabolism of fructose. In this study, using 13C NMR spectroscopy and [U-13C]fructose and [U-13C]glucose as stable 13C isotope tracers, we investigated the metabolic fate of fructose and glucose in B-cells. B-cells showed mitochondrial oxidation of fructose when administered alone, but showed diminished oxidation of fructose in the presence of glucose. On the other hand, fructose did not significantly affect the mitochondrial metabolism of glucose.",
keywords = "C isotope tracers, B-cells, Fructose metabolism, Mitochondrial oxidation, NMR spectroscopy",
author = "Ijare, {Omkar B.} and Baskin, {David S.} and Sharpe, {Martyn A.} and Kumar Pichumani",
note = "Funding Information: This study was supported by the Donna and Kenneth R. Peak Foundation, The Kenneth R. Peak Brain and Pituitary Tumor Treatment Center at Houston Methodist Hospital, The Houston Methodist Foundation, The Taub Foundation, The Pauline Sterne Wolff Foundation, The Veralan Foundation, and The John S. Dunn Foundation. We would like to thank Sophie Lopez for maintaining the cell lines and growing cells for this study. Funding Information: This study was supported by the Donna and Kenneth R. Peak Foundation , The Kenneth R. Peak Brain and Pituitary Tumor Treatment Center at Houston Methodist Hospital , The Houston Methodist Foundation , The Taub Foundation , The Pauline Sterne Wolff Foundation , The Veralan Foundation , and The John S. Dunn Foundation . We would like to thank Sophie Lopez for maintaining the cell lines and growing cells for this study. Publisher Copyright: {\textcopyright} 2018 Elsevier Inc. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",
year = "2018",
month = jul,
day = "1",
doi = "10.1016/j.ab.2018.04.003",
language = "English (US)",
volume = "552",
pages = "110--117",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press",
}