TY - JOUR
T1 - Metabolic Characteristics of 16HBE and A549 Cells Exposed to Different Surface Modified Gold Nanorods
AU - Liu, Zhigang
AU - Wang, Liming
AU - Zhang, Limin
AU - Wu, Xiaochun
AU - Nie, Guangjun
AU - Chen, Chunying
AU - Tang, Huiru
AU - Wang, Yulan
N1 - Funding Information:
The authors thank Dr. Caixiang Liu, Lingling Xu, Yinglu Ji, and Jinglong Tang for technical assistance in the experiments. This work was supported by grants from the National Basic Research Program of China (Grant 2012CB934004) and the National Natural Science Foundation of China (21675169, 21175149, 91439102, and 21375144).
Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2016/9/21
Y1 - 2016/9/21
N2 - Gold nanorods (AuNRs) have shown their great potential in cancer treatment due to their special physiochemical and optical properties, and the ease of surface modification. However, the molecular mechanism of biological effects induced by different surface modified AuNRs remains largely undetermined. Herein, this study for the first time systematically analyzed metabolic impacts of three surface modified AuNRs in cancer and noncancer cells detected by NMR and GC-FID/MS metabolomics and validated by molecular biological approach. It is found that positively and negatively charged AuNRs induce different metabolic consequences. Most importantly, it is found that the PEI-AuNRs display specific cytotoxicity to A549 cells while posing little impact on 16HBE cells. The cytotoxicity of PEI-AuNRs to A549 cells is manifested in large disruptions to the cell metabolisms, which affects energy metabolism, choline metabolism, the hexosamine biosynthesis pathway, and oxidative stress to cells. The results of this study provide comprehensive molecular information on the distinct biological effects of different surface modified AuNRs, and can be valuable in designing purpose-driven nanomaterials. Most importantly, this work highlights the potential of metabolomics coupled with molecular biological techniques in screening antitumor nanodrugs and revealing the molecular mechanism of their biological effects.
AB - Gold nanorods (AuNRs) have shown their great potential in cancer treatment due to their special physiochemical and optical properties, and the ease of surface modification. However, the molecular mechanism of biological effects induced by different surface modified AuNRs remains largely undetermined. Herein, this study for the first time systematically analyzed metabolic impacts of three surface modified AuNRs in cancer and noncancer cells detected by NMR and GC-FID/MS metabolomics and validated by molecular biological approach. It is found that positively and negatively charged AuNRs induce different metabolic consequences. Most importantly, it is found that the PEI-AuNRs display specific cytotoxicity to A549 cells while posing little impact on 16HBE cells. The cytotoxicity of PEI-AuNRs to A549 cells is manifested in large disruptions to the cell metabolisms, which affects energy metabolism, choline metabolism, the hexosamine biosynthesis pathway, and oxidative stress to cells. The results of this study provide comprehensive molecular information on the distinct biological effects of different surface modified AuNRs, and can be valuable in designing purpose-driven nanomaterials. Most importantly, this work highlights the potential of metabolomics coupled with molecular biological techniques in screening antitumor nanodrugs and revealing the molecular mechanism of their biological effects.
KW - biomedical applications
KW - cancer cell
KW - gold nanorods
KW - metabolomics
KW - surface modification
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U2 - 10.1002/adhm.201600164
DO - 10.1002/adhm.201600164
M3 - Article
C2 - 27391541
AN - SCOPUS:84978225991
VL - 5
SP - 2363
EP - 2375
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
SN - 2192-2640
IS - 18
ER -