Mesoporous silicon particles for sustained gene silencing

Nafis Hasan, Aman Mann, Mauro Ferrari, Takemi Tanaka

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

RNA interference (RNAi) is a powerful approach for silencing oncogenes; however, in vivo RNAi delivery has remained a major challenge due to lack of safe, efficient, and sustained delivery. Here, we describe a novel approach to overcome these limitations using mesoporous silicon particles loaded with nanoparticles (i.e., liposomes) containing small interfering RNA (siRNA) targeted against oncoprotein that contributes to cancer cell survival. This delivery method resulted in sustained gene silencing for at least 3 weeks with substantial reduction of tumor growth with no overt toxicities in two independent orthotopic mouse models of ovarian cancer following a single intravenous administration of mesoporous silicon particles loaded with liposomal EphA2-siRNA.

Original languageEnglish (US)
Title of host publicationOvarian Cancer
Subtitle of host publicationMethods and Protocols
EditorsAnastasia Malek, Oleg Tchernitsa
Pages481-493
Number of pages13
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1049
ISSN (Print)1064-3745

Keywords

  • Liposome
  • Mesoporous silicon particle
  • Multistage delivery
  • Nanoparticle
  • Ovarian cancer
  • RNA interference
  • Small interfering RNA
  • Sustained gene silencing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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