Mesenchymal stem cells as delivery vehicle of porphyrin loaded nanoparticles: Effective photoinduced in vitro killing of osteosarcoma

S. Duchi, G. Sotgiu, E. Lucarelli, M. Ballestri, B. Dozza, S. Santi, A. Guerrini, P. Dambruoso, S. Giannini, D. Donati, C. Ferroni, G. Varchi

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Mesenchymal stem cells (MSC) have the unique ability to home and engraft in tumor stroma. These features render them potentially a very useful tool as targeted delivery vehicles which can deliver therapeutic drugs to the tumor stroma. In the present study, we investigate whether fluorescent core-shell PMMA nanoparticles (FNPs) post-loaded with a photosensitizer, namely meso-tetrakis (4-sulfonatophenyl) porphyrin (TPPS) and uploaded by MSC could trigger osteosarcoma (OS) cell death in vitro upon specific photoactivation. In co-culture studies we demonstrate using laser confocal microscopy and time lapse imaging, that only after laser irradiation MSC loaded with photosensitizer- coated fluorescent NPs (TPPS@FNPs) undergo cell death and release reactive oxygen species (ROS) which are sufficient to trigger cell death of all OS cells in the culture. These results encourage further studies aimed at proving the efficacy of this novel tri-component system for PDT applications.

Original languageEnglish (US)
Pages (from-to)225-237
Number of pages13
JournalJournal of Controlled Release
Volume168
Issue number2
DOIs
StatePublished - Jun 10 2013

Keywords

  • Mesenchymal stem cells (MSC)
  • Nanoparticles (NPs)
  • Osteosarcoma (OS)
  • Photodynamic therapy (PDT)
  • Porphyrins (TPPS)

ASJC Scopus subject areas

  • Pharmaceutical Science

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