TY - JOUR
T1 - Mesenchymal stem cells and platelet-rich plasma enhance bone formation in sinus grafting
T2 - A histomorphometric study in minipigs
AU - Pieri, Francesco
AU - Lucarelli, Enrico
AU - Corinaldesi, Giuseppe
AU - Iezzi, Giovanna
AU - Piattelli, Adriano
AU - Giardino, Roberto
AU - Bassi, Massimo
AU - Donati, Davide
AU - Marchetti, Claudio
PY - 2008/6
Y1 - 2008/6
N2 - Objectives: Autologous, allogenic, and alloplastic materials for sinus augmentation have specific drawbacks, which has stimulated an ongoing search for new materials and tissue-engineering constructs. We investigated whether mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) seeded on a fluorohydroxyapatite (FH) scaffold can improve bone formation and bone-to-implant contact (BIC) in maxillary sinus grafting. Material and Methods: Bilateral sinus augmentation procedures were performed in eight minipigs. MSCs, PRP, and FH scaffold (test site) or FH alone (control site) were grafted in each maxillary sinus. Distal to the osteotomy, one dental implant per sinus was placed in the grafting material through the facial sinus wall. The animals were killed 3 months after grafting, and block sections of the implant sites were harvested and prepared for histomorphometric analysis. Results: After 12 weeks, a significant increase in bone formation occurred in the test sites compared with the control sites (42.51% versus 18.98%; p=0.001). In addition, BIC was significantly greater in the test sites compared with the control sites in the regenerated area (23.71% versus 6.63%; p=0.028). Conclusions: These findings show that sinus augmentation with MSCs-PRP, combined with FH may enhance bone formation and osseointegration of dental implants compared with FH alone in minipigs.
AB - Objectives: Autologous, allogenic, and alloplastic materials for sinus augmentation have specific drawbacks, which has stimulated an ongoing search for new materials and tissue-engineering constructs. We investigated whether mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) seeded on a fluorohydroxyapatite (FH) scaffold can improve bone formation and bone-to-implant contact (BIC) in maxillary sinus grafting. Material and Methods: Bilateral sinus augmentation procedures were performed in eight minipigs. MSCs, PRP, and FH scaffold (test site) or FH alone (control site) were grafted in each maxillary sinus. Distal to the osteotomy, one dental implant per sinus was placed in the grafting material through the facial sinus wall. The animals were killed 3 months after grafting, and block sections of the implant sites were harvested and prepared for histomorphometric analysis. Results: After 12 weeks, a significant increase in bone formation occurred in the test sites compared with the control sites (42.51% versus 18.98%; p=0.001). In addition, BIC was significantly greater in the test sites compared with the control sites in the regenerated area (23.71% versus 6.63%; p=0.028). Conclusions: These findings show that sinus augmentation with MSCs-PRP, combined with FH may enhance bone formation and osseointegration of dental implants compared with FH alone in minipigs.
KW - Dental implants
KW - Platelet-rich plasma
KW - Sinus grafting
KW - Stem cells
KW - Tissue engineering
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U2 - 10.1111/j.1600-051X.2008.01220.x
DO - 10.1111/j.1600-051X.2008.01220.x
M3 - Article
C2 - 18422697
AN - SCOPUS:43449129449
SN - 0303-6979
VL - 35
SP - 539
EP - 546
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
IS - 6
ER -