Members of the nuclear factor 1 family reduce the transcriptional potential of the nuclear receptor LXRα promoter

Knut R. Steffensen; Elin Holter; Kari A.R. Tobin; Steeve Leclerc; Jan Åke Gustafsson; Sylvain L. Guérin; Winnie Eskild

doi:10.1006/bbrc.2001.6078

Members of the nuclear factor 1 family reduce the transcriptional potential of the nuclear receptor LXRα promoter

Knut R. Steffensen, Elin Holter, Kari A.R. Tobin, Steeve Leclerc, Jan Åke Gustafsson, Sylvain L. Guérin, Winnie Eskild

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Expression of the LXRα nuclear receptor in liver is predicted to affect cholesterol and lipid metabolism. Here we show that a short fragment from the LXRα gene promoter spanning the region from -144 to +43 relative to the mRNA initiation site can drive transcription of a reporter gene. Under basal conditions, in vitro DNase I footprinting demonstrated interaction between nuclear proteins and an NF1 recognition site in close vicinity to the transcriptional initiation. Both supershift, mutational analyses in EMSA and transfections provided evidence that the NF1 (nuclear factor I) transcription factor interacts with the LXRα promoter. All four members of the NF1 family were found to suppress the transcriptional activity indicating a general inhibitory effect on LXRα expression. A similar regulation by NF1 was also observed when using a fragment from the LXRα promoter extending up to position -3033 therefore giving the inhibitory effect of NF1 a significant impact on LXRα gene expression.

Original language	English (US)
Pages (from-to)	1262-1267
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	289
Issue number	5
DOIs	https://doi.org/10.1006/bbrc.2001.6078
State	Published - Dec 21 2001

Keywords

Gene expression
Nuclear factor 1
Nuclear receptors
Transcription
Transcription factors

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1006/bbrc.2001.6078

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title = "Members of the nuclear factor 1 family reduce the transcriptional potential of the nuclear receptor LXRα promoter",

abstract = "Expression of the LXRα nuclear receptor in liver is predicted to affect cholesterol and lipid metabolism. Here we show that a short fragment from the LXRα gene promoter spanning the region from -144 to +43 relative to the mRNA initiation site can drive transcription of a reporter gene. Under basal conditions, in vitro DNase I footprinting demonstrated interaction between nuclear proteins and an NF1 recognition site in close vicinity to the transcriptional initiation. Both supershift, mutational analyses in EMSA and transfections provided evidence that the NF1 (nuclear factor I) transcription factor interacts with the LXRα promoter. All four members of the NF1 family were found to suppress the transcriptional activity indicating a general inhibitory effect on LXRα expression. A similar regulation by NF1 was also observed when using a fragment from the LXRα promoter extending up to position -3033 therefore giving the inhibitory effect of NF1 a significant impact on LXRα gene expression.",

keywords = "Gene expression, Nuclear factor 1, Nuclear receptors, Transcription, Transcription factors",

author = "Steffensen, {Knut R.} and Elin Holter and Tobin, {Kari A.R.} and Steeve Leclerc and Gustafsson, {Jan {\AA}ke} and Gu{\'e}rin, {Sylvain L.} and Winnie Eskild",

note = "Funding Information: We thank Dr. Naoko Tanese and Dr. Richard Gronostajski, for kindly providing us with the NF1 antiserum and the NF1 expression plasmids, respectively. K. R. Steffensen is supported by Grant A97030/002 from The Norwegian Cancer Society. S. L. Gu{\'e}rin is a Scholar from the Fonds de la Recherche en Sant{\'e} du Qu{\'e}bec (FRSQ). Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

year = "2001",

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language = "English (US)",

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AU - Tobin, Kari A.R.

AU - Leclerc, Steeve

AU - Gustafsson, Jan Åke

AU - Guérin, Sylvain L.

AU - Eskild, Winnie

N1 - Funding Information: We thank Dr. Naoko Tanese and Dr. Richard Gronostajski, for kindly providing us with the NF1 antiserum and the NF1 expression plasmids, respectively. K. R. Steffensen is supported by Grant A97030/002 from The Norwegian Cancer Society. S. L. Guérin is a Scholar from the Fonds de la Recherche en Santé du Québec (FRSQ). Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2001/12/21

Y1 - 2001/12/21

N2 - Expression of the LXRα nuclear receptor in liver is predicted to affect cholesterol and lipid metabolism. Here we show that a short fragment from the LXRα gene promoter spanning the region from -144 to +43 relative to the mRNA initiation site can drive transcription of a reporter gene. Under basal conditions, in vitro DNase I footprinting demonstrated interaction between nuclear proteins and an NF1 recognition site in close vicinity to the transcriptional initiation. Both supershift, mutational analyses in EMSA and transfections provided evidence that the NF1 (nuclear factor I) transcription factor interacts with the LXRα promoter. All four members of the NF1 family were found to suppress the transcriptional activity indicating a general inhibitory effect on LXRα expression. A similar regulation by NF1 was also observed when using a fragment from the LXRα promoter extending up to position -3033 therefore giving the inhibitory effect of NF1 a significant impact on LXRα gene expression.

AB - Expression of the LXRα nuclear receptor in liver is predicted to affect cholesterol and lipid metabolism. Here we show that a short fragment from the LXRα gene promoter spanning the region from -144 to +43 relative to the mRNA initiation site can drive transcription of a reporter gene. Under basal conditions, in vitro DNase I footprinting demonstrated interaction between nuclear proteins and an NF1 recognition site in close vicinity to the transcriptional initiation. Both supershift, mutational analyses in EMSA and transfections provided evidence that the NF1 (nuclear factor I) transcription factor interacts with the LXRα promoter. All four members of the NF1 family were found to suppress the transcriptional activity indicating a general inhibitory effect on LXRα expression. A similar regulation by NF1 was also observed when using a fragment from the LXRα promoter extending up to position -3033 therefore giving the inhibitory effect of NF1 a significant impact on LXRα gene expression.

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KW - Transcription factors

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Members of the nuclear factor 1 family reduce the transcriptional potential of the nuclear receptor LXRα promoter

Abstract

Keywords

ASJC Scopus subject areas

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