TY - JOUR
T1 - Mechanism of release of gastric luminal somatostatin-like immunoreactivity in response to pentagastrin and sham feeding in man
AU - Ozden, A.
AU - Degertekin, H.
AU - Yeginsu, A. O.
AU - Ertan, A.
N1 - Funding Information:
This study was supported by the Gastroenterology Section Research Fund 530569. The authors would like to acknowledge the biostatistical analysis of Dr. Janet Rice, the editorial help of Dr. Gillian F. Brown and the secretarial assistance of Miss April Thornton. This study was presented in part at the meeting of the Southern Society for Clinical Investigation in New Orleans, La., USA.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1987
Y1 - 1987
N2 - We studied in five healthy volunteers whether the cholinergic pathway regulated the secretion of gastric intraluminal somatostatin-like immunoreactivity (SLI) in response to stimuli of pentagastrin infusion (0.9 μg/kg/h, intravenously) and sham feeding. We measured gastric secretory volume, hydrogen ion output, and SLI at base line, during pentagastrin infusion, after sham feeding, and after applications of atropine (0.0, 0.7, 7.0 μg/kg, intramuscularly) given before pentagastrin and sham feeding. The stimuli were given randomly, at separate times on different days. After each stimulus, eight 15-min gastric juice collections were made; samples were adjusted to pH 7, pepstatin-A and aprotinin were added, and samples were extracted with acetone to determine SLI by radioimmunoassay. Pentagastrin and sham feeding significantly increased gastric luminal SLI secretion, which appeared to correlate with the increases in volume and acid output. Atropine at 7 μg/kg significantly suppressed gastric volume, acid, and SLI outputs stimulated by sham feeding; however, responses to pentagastrin stimulation remained unchanged. To conclude, the cholinergic mechanism regulates gastric intraluminal SLI response to sham feeding but not to pentagastrin infusion.
AB - We studied in five healthy volunteers whether the cholinergic pathway regulated the secretion of gastric intraluminal somatostatin-like immunoreactivity (SLI) in response to stimuli of pentagastrin infusion (0.9 μg/kg/h, intravenously) and sham feeding. We measured gastric secretory volume, hydrogen ion output, and SLI at base line, during pentagastrin infusion, after sham feeding, and after applications of atropine (0.0, 0.7, 7.0 μg/kg, intramuscularly) given before pentagastrin and sham feeding. The stimuli were given randomly, at separate times on different days. After each stimulus, eight 15-min gastric juice collections were made; samples were adjusted to pH 7, pepstatin-A and aprotinin were added, and samples were extracted with acetone to determine SLI by radioimmunoassay. Pentagastrin and sham feeding significantly increased gastric luminal SLI secretion, which appeared to correlate with the increases in volume and acid output. Atropine at 7 μg/kg significantly suppressed gastric volume, acid, and SLI outputs stimulated by sham feeding; however, responses to pentagastrin stimulation remained unchanged. To conclude, the cholinergic mechanism regulates gastric intraluminal SLI response to sham feeding but not to pentagastrin infusion.
KW - Gastric
KW - Intraluminal
KW - Pentagastrin
KW - Sham feeding
KW - Somatostatin-like immunoreactivity
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U2 - 10.3109/00365528708996473
DO - 10.3109/00365528708996473
M3 - Article
C2 - 3433015
AN - SCOPUS:0023611667
SN - 0036-5521
VL - 22
SP - 1257
EP - 1262
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
IS - 10
ER -