TY - JOUR
T1 - Mechanical Cues for Triggering and Regulating Cellular Movement Selectively at the Single-Cell Level
AU - Ogorodnik, Evgeny
AU - Karsai, Arpad
AU - Liu, Ying X.
AU - Di Lucente, Jacopo
AU - Huang, Yuqi
AU - Keel, Terell
AU - Haudenschild, Dominik R.
AU - Jin, Lee Way
AU - Liu, Gang Yu
N1 - Publisher Copyright:
© 2023 American Chemical Society.
PY - 2023/2/2
Y1 - 2023/2/2
N2 - Cell motility plays important roles in many biophysical and physiological processes ranging from in vitro biomechanics, wound healing, to cancer metastasis. This work introduces a new means to trigger and regulate motility individually using transient mechanical stimulus applied to designated cells. Using BV2 microglial cells, our investigations indicate that motility can be reproducibly and reliably initiated using mechanical compression of the cells. The location and magnitude of the applied force impact the movement of the cell. Based on observations from this investigation and current knowledge of BV2 cellular motility, new physical insights are revealed into the underlying mechanism of force-induced single cellular movement. The process involves high degrees of myosin activation to repair actin cortex breakages induced by the initial mechanical compression, which leads to focal adhesion degradation, lamellipodium detachment, and finally, cell polarization and movement. Modern technology enables accurate control over force magnitude and location of force delivery, thus bringing us closer to programming cellular movement at the single-cell level. This approach is of generic importance to other cell types beyond BV2 cells and has the intrinsic advantages of being transient, non-toxic, and non-destructive, thus exhibiting high translational potentials including mechano-based therapy.
AB - Cell motility plays important roles in many biophysical and physiological processes ranging from in vitro biomechanics, wound healing, to cancer metastasis. This work introduces a new means to trigger and regulate motility individually using transient mechanical stimulus applied to designated cells. Using BV2 microglial cells, our investigations indicate that motility can be reproducibly and reliably initiated using mechanical compression of the cells. The location and magnitude of the applied force impact the movement of the cell. Based on observations from this investigation and current knowledge of BV2 cellular motility, new physical insights are revealed into the underlying mechanism of force-induced single cellular movement. The process involves high degrees of myosin activation to repair actin cortex breakages induced by the initial mechanical compression, which leads to focal adhesion degradation, lamellipodium detachment, and finally, cell polarization and movement. Modern technology enables accurate control over force magnitude and location of force delivery, thus bringing us closer to programming cellular movement at the single-cell level. This approach is of generic importance to other cell types beyond BV2 cells and has the intrinsic advantages of being transient, non-toxic, and non-destructive, thus exhibiting high translational potentials including mechano-based therapy.
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U2 - 10.1021/acs.jpcb.2c06461
DO - 10.1021/acs.jpcb.2c06461
M3 - Article
C2 - 36652348
AN - SCOPUS:85146549878
SN - 1520-6106
VL - 127
SP - 866
EP - 873
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
IS - 4
ER -