The measurement and potential technological significance of in vivo missnse errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the α subunit of E coli tryptophan synthetase and bacterial luciferase are discussed, as well as the prospects for further development of this method.
- tryptophan synthetase
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