Measurement of translational accuracy in vivo: missense reporting using inactive enzyme mutants

B. Cornut; R. C. Willson

doi:10.1016/0300-9084(91)90192-4

Measurement of translational accuracy in vivo: missense reporting using inactive enzyme mutants

B. Cornut, R. C. Willson

Research output: Contribution to journal › Article

6 Scopus citations

Abstract

The measurement and potential technological significance of in vivo missnse errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the α subunit of E coli tryptophan synthetase and bacterial luciferase are discussed, as well as the prospects for further development of this method.

Original language	English (US)
Pages (from-to)	1567-1572
Number of pages	6
Journal	Biochimie
Volume	73
Issue number	12
DOIs	https://doi.org/10.1016/0300-9084(91)90192-4
State	Published - Dec 1991

Keywords

luciferase
missense
mistranslation
ribosome
tryptophan synthetase

ASJC Scopus subject areas

Biochemistry

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title = "Measurement of translational accuracy in vivo: missense reporting using inactive enzyme mutants",

abstract = "The measurement and potential technological significance of in vivo missnse errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the α subunit of E coli tryptophan synthetase and bacterial luciferase are discussed, as well as the prospects for further development of this method.",

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AU - Willson, R. C.

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AB - The measurement and potential technological significance of in vivo missnse errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the α subunit of E coli tryptophan synthetase and bacterial luciferase are discussed, as well as the prospects for further development of this method.

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KW - missense

KW - mistranslation

KW - ribosome

KW - tryptophan synthetase

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