Abstract
The measurement and potential technological significance of in vivo missnse errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the α subunit of E coli tryptophan synthetase and bacterial luciferase are discussed, as well as the prospects for further development of this method.
Original language | English (US) |
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Pages (from-to) | 1567-1572 |
Number of pages | 6 |
Journal | Biochimie |
Volume | 73 |
Issue number | 12 |
DOIs | |
State | Published - Dec 1991 |
Keywords
- luciferase
- missense
- mistranslation
- ribosome
- tryptophan synthetase
ASJC Scopus subject areas
- Biochemistry