Measurement of translational accuracy in vivo: missense reporting using inactive enzyme mutants

B. Cornut, R. C. Willson

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The measurement and potential technological significance of in vivo missnse errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the α subunit of E coli tryptophan synthetase and bacterial luciferase are discussed, as well as the prospects for further development of this method.

Original languageEnglish (US)
Pages (from-to)1567-1572
Number of pages6
JournalBiochimie
Volume73
Issue number12
DOIs
StatePublished - Dec 1991

Keywords

  • luciferase
  • missense
  • mistranslation
  • ribosome
  • tryptophan synthetase

ASJC Scopus subject areas

  • Biochemistry

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