Abstract
We have recently reported a new and rapid assay to measure plasma holotranscobalamin II (holoTC II) as a means of exploring vitamin B12 status. In order to further evaluate the significance of plasma holoTC II in determining tissue cobalamin, we have chosen the red blood cell-vitamin B12 (RBC-B12) assay as a measure of tissue vitamin B12 content and studied the relationship between RBC-B12 and plasma holoTC II levels. Plasma holoTC II and RBC-B12 concentrations were concomitantly assayed in 20 hematologically normal controls and cancer patients. In our groups of controls, the mean value of RBC-B12 was determined as 241 ± 51 pg/ml of packed erythrocytes (PE) with a range varying from 180 to 355 pg/ml PE. Preliminary results obtained in 32 cancer patients revealed lower holoTC II and RBC-B12 levels than the control group and a required threshold value of 70 pg/ml of holoTC II in order to maintain a normal RBC-B12 greater than 180 pg/ml PE.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 226-229 |
| Number of pages | 4 |
| Journal | American Journal of Hematology |
| Volume | 43 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jul 1993 |
Keywords
- cobalamin
- cobalamin deficiency
- negative B12 balance
ASJC Scopus subject areas
- Hematology
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