Measurement of red blood cell‐vitamin B12: A study of the correlation between intracellular B12 content and concentrations of plasma holotranscobalamin II

G. Tisman, T. Vu, J. Amin, G. Luszko, Malcolm Brenner, M. Ramos, V. Flener, V. Cordts, R. Bateman, S. Malkin, T. Browder

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We have recently reported a new and rapid assay to measure plasma holotranscobalamin II (holoTC II) as a means of exploring vitamin B12 status. In order to further evaluate the significance of plasma holoTC II in determining tissue cobalamin, we have chosen the red blood cell-vitamin B12 (RBC-B12) assay as a measure of tissue vitamin B12 content and studied the relationship between RBC-B12 and plasma holoTC II levels. Plasma holoTC II and RBC-B12 concentrations were concomitantly assayed in 20 hematologically normal controls and cancer patients. In our groups of controls, the mean value of RBC-B12 was determined as 241 ± 51 pg/ml of packed erythrocytes (PE) with a range varying from 180 to 355 pg/ml PE. Preliminary results obtained in 32 cancer patients revealed lower holoTC II and RBC-B12 levels than the control group and a required threshold value of 70 pg/ml of holoTC II in order to maintain a normal RBC-B12 greater than 180 pg/ml PE.

Original languageEnglish (US)
Pages (from-to)226-229
Number of pages4
JournalAmerican Journal of Hematology
Volume43
Issue number3
DOIs
StatePublished - Jan 1 1993

Keywords

  • cobalamin
  • cobalamin deficiency
  • negative B12 balance

ASJC Scopus subject areas

  • Hematology

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