Measurement of plasma prostaglandin E2 using capillary gas chromatography negative ion chemical ionization mass spectrometry

B. J. Smith, D. A. Herold, R. M. Ross, F. G. Marquis, R. L. Bertholf, C. R. Ayers, M. R. Wills, J. Savory

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A stable isotope dilution assay for the measurement of plasma prostaglandin E2 (PGE2) employing capillary column gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS) is described. PGE2 was extracted from plasma using C18 and silica SEP-PAKS. Further purification and separation was accomplished by thin layer chromatography. The prostaglandin was analyzed as its pentafluorobenzyl ester-methoxime-trimethylsilyl ether, using fragment ions at m/e 524 (protium) and m/e 528 (deuterium) for quantitation. The mean plasma concentration of PGE2 determined in 8 healthy volunteers was 2.8 ± 2.0 pg/ml.

Original languageEnglish (US)
Pages (from-to)73-86
Number of pages14
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume40
Issue number1
StatePublished - 1983

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Pharmacology
  • Pharmacology, Toxicology and Pharmaceutics(all)

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