Measurement of calcium release due to inositol trisphosphate receptors in skeletal muscle

Mariana Casas, Francisco Altamirano, Enrique Jaimovich

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

Calcium transients elicited by IP 3 receptors upon electrical stimulation of skeletal muscle cells (slow calcium signals) are often hard to visualize due to their relatively small amplitude compared to the large transient originated from ryanodine receptors associated to excitation- contraction coupling. The study of slow calcium transients, however, is relevant due to their function in regulation of muscle gene expression and in the process of excitation-transcription coupling. Discussed here are the procedures used to record slow calcium signals from both cultured mouse myotubes and from cultured adult skeletal muscle fibers.

Original languageEnglish (US)
Title of host publicationMyogenesis
Subtitle of host publicationMethods and Protocols
EditorsJoseph DiMario
Pages383-393
Number of pages11
DOIs
StatePublished - Jan 2 2012

Publication series

NameMethods in Molecular Biology
Volume798
ISSN (Print)1064-3745

Keywords

  • Calcium dyes
  • Cultured muscle fibers
  • Electrical stimulation
  • Gene expression
  • Myotubes
  • Slow calcium signals

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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