Measurement and significance of antibodies against GM1ganglioside Report of a workshop, 18 April 1989, Chicago, IL, U.S.A.

Donald M. Marcus; Norman Latov; Bart P. Hsi; Baiba K. Gillard; Jack Antel; Stanley Appel; Lanfranco Callegaro; Genevieve Daune; Baiba Gillard; Norman Latoy; Donald Marcus; Eduardo Nobile-Orazio; Alan Pestronk; Jacques Portoukalian; Richard Quarles; Edgar Salazar-Grueso; Larry Schneider; Michael Shy; Andreas Steck

doi:10.1016/0165-5728(89)90144-6

Measurement and significance of antibodies against G_M1ganglioside Report of a workshop, 18 April 1989, Chicago, IL, U.S.A.

Donald M. Marcus, Norman Latov, Bart P. Hsi, Baiba K. Gillard, Jack Antel, Stanley Appel, Lanfranco Callegaro, Genevieve Daune, Baiba Gillard, Norman Latoy, Donald Marcus, Eduardo Nobile-Orazio, Alan Pestronk, Jacques Portoukalian, Richard Quarles, Edgar Salazar-Grueso, Larry Schneider, Michael Shy, Andreas Steck

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

Twelve laboratories from the United States, Canada, France, Italy and Switzerland participated in a workshop to compare assay used to measure anti-G_M1 antibodies, and to discuss the clinical significance of these antibodies. A panel of test samples containing varying amounts of anti-G_M1 antibody was prepared by mixing varied proportions of normal serum with a serum containing a monoclonal IgM antibody that bound G_M1 ganglioside. Enzyme-linked immunosorbent assay (ELISA) data were supplied by eight laboratories and ten laboratories classified the sera as negative, weakly of strongly positive. Most laboratories correctly identified the two samples that contained the highest quantities of antibody, but there was considerable disagreement on the classification of the three samples with moderate or small.

Original language	English (US)
Pages (from-to)	255-259
Number of pages	5
Journal	Journal of Neuroimmunology
Volume	25
Issue number	2-3
DOIs	https://doi.org/10.1016/0165-5728(89)90144-6
State	Published - Jan 1 1989

Keywords

Anti-ganglioside
Enzyme-linked immunosorbent assay
Neuropathy

ASJC Scopus subject areas

Neurology
Clinical Neurology
Immunology and Allergy
Immunology

Divisions

Geriatrics

Access to Document

10.1016/0165-5728(89)90144-6

Cite this

Marcus, D. M., Latov, N., Hsi, B. P., Gillard, B. K., Antel, J., Appel, S., Callegaro, L., Daune, G., Gillard, B., Latoy, N., Marcus, D., Nobile-Orazio, E., Pestronk, A., Portoukalian, J., Quarles, R., Salazar-Grueso, E., Schneider, L., Shy, M., & Steck, A. (1989). Measurement and significance of antibodies against G_M1ganglioside Report of a workshop, 18 April 1989, Chicago, IL, U.S.A. Journal of Neuroimmunology, 25(2-3), 255-259. https://doi.org/10.1016/0165-5728(89)90144-6

Marcus, DM, Latov, N, Hsi, BP, Gillard, BK, Antel, J, Appel, S, Callegaro, L, Daune, G, Gillard, B, Latoy, N, Marcus, D, Nobile-Orazio, E, Pestronk, A, Portoukalian, J, Quarles, R, Salazar-Grueso, E, Schneider, L, Shy, M & Steck, A 1989, 'Measurement and significance of antibodies against G_M1ganglioside Report of a workshop, 18 April 1989, Chicago, IL, U.S.A.', Journal of Neuroimmunology, vol. 25, no. 2-3, pp. 255-259. https://doi.org/10.1016/0165-5728(89)90144-6

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abstract = "Twelve laboratories from the United States, Canada, France, Italy and Switzerland participated in a workshop to compare assay used to measure anti-GM1 antibodies, and to discuss the clinical significance of these antibodies. A panel of test samples containing varying amounts of anti-GM1 antibody was prepared by mixing varied proportions of normal serum with a serum containing a monoclonal IgM antibody that bound GM1 ganglioside. Enzyme-linked immunosorbent assay (ELISA) data were supplied by eight laboratories and ten laboratories classified the sera as negative, weakly of strongly positive. Most laboratories correctly identified the two samples that contained the highest quantities of antibody, but there was considerable disagreement on the classification of the three samples with moderate or small.",

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AU - Marcus, Donald M.

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AU - Gillard, Baiba K.

AU - Antel, Jack

AU - Appel, Stanley

AU - Callegaro, Lanfranco

AU - Daune, Genevieve

AU - Gillard, Baiba

AU - Latoy, Norman

AU - Marcus, Donald

AU - Nobile-Orazio, Eduardo

AU - Pestronk, Alan

AU - Portoukalian, Jacques

AU - Quarles, Richard

AU - Salazar-Grueso, Edgar

AU - Schneider, Larry

AU - Shy, Michael

AU - Steck, Andreas

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AB - Twelve laboratories from the United States, Canada, France, Italy and Switzerland participated in a workshop to compare assay used to measure anti-GM1 antibodies, and to discuss the clinical significance of these antibodies. A panel of test samples containing varying amounts of anti-GM1 antibody was prepared by mixing varied proportions of normal serum with a serum containing a monoclonal IgM antibody that bound GM1 ganglioside. Enzyme-linked immunosorbent assay (ELISA) data were supplied by eight laboratories and ten laboratories classified the sera as negative, weakly of strongly positive. Most laboratories correctly identified the two samples that contained the highest quantities of antibody, but there was considerable disagreement on the classification of the three samples with moderate or small.

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