Twelve laboratories from the United States, Canada, France, Italy and Switzerland participated in a workshop to compare assay used to measure anti-GM1 antibodies, and to discuss the clinical significance of these antibodies. A panel of test samples containing varying amounts of anti-GM1 antibody was prepared by mixing varied proportions of normal serum with a serum containing a monoclonal IgM antibody that bound GM1 ganglioside. Enzyme-linked immunosorbent assay (ELISA) data were supplied by eight laboratories and ten laboratories classified the sera as negative, weakly of strongly positive. Most laboratories correctly identified the two samples that contained the highest quantities of antibody, but there was considerable disagreement on the classification of the three samples with moderate or small.
- Enzyme-linked immunosorbent assay
ASJC Scopus subject areas
- Clinical Neurology
- Immunology and Allergy