Matrix metalloproteinase-9 maps to the distal end of chromosome 2 in the mouse

Kevin J. Leco, Mark B. Harvey, Aileen Hogan, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Dylan R. Edwards, Gilbert A. Schultz

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The activity and expression of matrix metalloproteinase-9/gelatinase B (MMP-9), an enzyme implicated in the implantation process in mice, was investigated in normal and parthenogenetic blastocyst outgrowths. Conditioned media from parthenogenetic blastocysts after 4 days of culture had reduced levels of MMP-9 activity compared to conditioned medium from normal outgrowths. Levels of MMP-9 mRNA assayed by reverse transcription-polymerase chain reaction methods were also reduced in parthenogenetic blastocysts compared to normal outgrowths. Genetic mapping studies showed that Mmp9 maps to the distal end of chromosome 2 near the proximal boundary of a region affected by genomic imprinting. Both parental alleles of Mmp9, however, are expressed in 11.5-day embryos derived from interspecific crosses of Mus musculus and Mus spretus. Thus, loss of MMP-9 activity in parthenogenetic blastocysts does not appear to be due to imprinting but, rather, due to a defect of trophoblast giant cell proliferation and differentiation.

Original languageEnglish (US)
Pages (from-to)55-60
Number of pages6
JournalDevelopmental Genetics
Volume21
Issue number1
DOIs
StatePublished - Sep 22 1997

Keywords

  • Imprinting
  • Parthenogenetic embryos
  • Trophoblast

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics

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