Mapping cyclic stretch in the postpneumonectomy murine lung

Nenad Filipovic, Barry C. Gibney, Milos Kojic, Dalibor Nikolic, Velibor Isailovic, Alexandra Ysasi, Moritz A. Konerding, Steven J. Mentzer, Akira Tsuda

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

In many mammalian species, the removal of one lung [pneumonectomy (PNX)] is associated with the compensatory growth of the remaining lung. To investigate the hypothesis that parenchymal deformation may trigger lung regeneration, we used respiratorygated micro-computed tomography scanning to create threedimensional finite-element geometric models of the murine cardiac lobe with cyclic breathing. Models were constructed of respiratorygated micro-computed tomography scans pre-PNX and 24 h post- PNX. The computational models demonstrated that the maximum stretch ratio map was patchy and heterogeneous, particularly in subpleural, juxta-diaphragmatic, and cephalad regions of the lobe. In these parenchymal regions, the material line segments at peak inspiration were frequently two- to fourfold greater after PNX; some regions of the post-PNX cardiac lobe demonstrated parenchymal compression at peak inspiration. Similarly, analyses of parenchymal maximum shear strain demonstrated heterogeneous regions of mechanical stress with focal regions demonstrating a threefold increase in shear strain after PNX. Consistent with previously identified growth patterns, these subpleural regions of enhanced stretch and shear strain are compatible with a mechanical signal, likely involving cyclic parenchymal stretch, triggering lung growth.

Original languageEnglish (US)
Pages (from-to)1370-1378
Number of pages9
JournalJournal of applied physiology
Volume115
Issue number9
DOIs
StatePublished - Nov 1 2013

Keywords

  • Finite element
  • Image registration
  • Lung
  • Pneumonectomy
  • Regeneration

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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