Manipulation of intra- and extracellular ph in spontaneous canine tumours by use of hyperglycaemia

D. M. Prescott, H. C. Charles, H. D. Sostman, R. L. Page, D. E. Thrall, D. Moore, J. R. Oleson, M. W. Dewhirst

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We evaluated the use of hyperglycaemia to reduce tumour pH in dog with spontaneous tumours. Dogs were randomized to two groups: control and glucose. Intravenous administration of 20 % glucose was used to induce and maintain hyperglycaemia. Extra-and intracellular tumour pH were measured using interstitial pH microelectrodes and 31P-MRs, respectively. During the administration of glucose, the mean (± SEM) blood glucose concentration was 419.8 (± 32.8) and 121.1 (± 8.0) mg/dl for the glucose and control groups, respectively. The mean extracellular tumour pH before and following 90 min of hyperglycaemia was 7.15 (± 0.08) and 7.15 (± 0.09). During consecutive measurements, intracellular tumour pH did not change significantly for the control group or the group subjected to hyperglycaemic manipulation. In contradistinction to previous rodent studies, our results demonstrate that hyperglycaemia alone is not sufficient to manipulate either intra- (pH(i)) or extracellular (pH(e)) hydrogen ion concentration in spontaneous canine soft tissue tumours.

Original languageEnglish (US)
Pages (from-to)745-754
Number of pages10
JournalInternational Journal of Hyperthermia
Volume9
Issue number5
DOIs
StatePublished - 1993

Keywords

  • Canine
  • Hyperglycaemia
  • Hyperthermia
  • Tumour ph

ASJC Scopus subject areas

  • Cancer Research
  • Radiological and Ultrasound Technology

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