TY - JOUR
T1 - Mammalian cell/vaccinia virus expression vectors with increased stability of retroviral sequences in Escherichia coli
T2 - production of feline immunodeficiency virus envelope protein
AU - Wang, Rong Fu
AU - Mullins, James I.
N1 - Funding Information:
We thank Drs. D.L. Sodora for provision of the FIV en v gene, E.A. Hoover for the cat sera, N. Arai for pcDL-SRa296, B. Moss for plasmid pTM3, and J. Arthos and J. Wissink for pCLDN and pCIALDN and discussions. This work was supported by the Pediatric AIDS Foundation (555004-11-AR) and the US Public Health Service (AI32885). R.W. was supported in part by the Marian and Harold Stern Foundation.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1995/2/14
Y1 - 1995/2/14
N2 - Many eukaryotic DNA sequences, especially lenti-retrovirus proviral genomes and their env genes, are unstable when cloned in high-copy-number plasmids in Escherichia coli. Stability can be increased by the use of low-copy-number vectors, although plasmid yields are low. Vectors are described here that contain the intermediate-copy-number P15A ori for cloning, stable propagation and higher-yield production of plasmid DNA in E. coli, and the f1 ori for propagation as single-stranded phage. These vectors also have the capacity to direct high-yield production of protein in mammalian cells, and the option of incorporation into and expression via a T7 promoter in vaccinia virus. The SRα promoter, encephalomyocarditis (EMC) virus untranslated leader sequence, and poly(A) signal sequence serve as a high-yield mammalian cell expression cassette without the requirement for mRNA capping. A polyhistidine sequence is available at the 3′ end of the cassette to facilitate chromatographic purification of protein, neo and gpt genes were included in some vectors to serve as selectable markers, and the dhfr gene was included in one to achieve gene amplification in mammalian cells. Dicistronic mRNAs can be generated by insertion of coding sequences up and downstream from the EMC leader. The utility of these vectors was shown through expression of feline immunodeficiency virus (FIV) Env protein, in conjunction with the tissue plasminogen activator (tPA) leader sequence.
AB - Many eukaryotic DNA sequences, especially lenti-retrovirus proviral genomes and their env genes, are unstable when cloned in high-copy-number plasmids in Escherichia coli. Stability can be increased by the use of low-copy-number vectors, although plasmid yields are low. Vectors are described here that contain the intermediate-copy-number P15A ori for cloning, stable propagation and higher-yield production of plasmid DNA in E. coli, and the f1 ori for propagation as single-stranded phage. These vectors also have the capacity to direct high-yield production of protein in mammalian cells, and the option of incorporation into and expression via a T7 promoter in vaccinia virus. The SRα promoter, encephalomyocarditis (EMC) virus untranslated leader sequence, and poly(A) signal sequence serve as a high-yield mammalian cell expression cassette without the requirement for mRNA capping. A polyhistidine sequence is available at the 3′ end of the cassette to facilitate chromatographic purification of protein, neo and gpt genes were included in some vectors to serve as selectable markers, and the dhfr gene was included in one to achieve gene amplification in mammalian cells. Dicistronic mRNAs can be generated by insertion of coding sequences up and downstream from the EMC leader. The utility of these vectors was shown through expression of feline immunodeficiency virus (FIV) Env protein, in conjunction with the tissue plasminogen activator (tPA) leader sequence.
KW - His
KW - Recombinant DNA
KW - shuttle vector
KW - tPA leader
KW - vaccinia virus
UR - http://www.scopus.com/inward/record.url?scp=0028816801&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028816801&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(94)00743-C
DO - 10.1016/0378-1119(94)00743-C
M3 - Article
C2 - 7875588
AN - SCOPUS:0028816801
VL - 153
SP - 197
EP - 202
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -