Magnitude of IFN-γ production in HIV-1-infected children is associated with virus suppression

James M. Reuben, Bang Ning Lee, Mary Paul, Mark W. Kline, Stanley G. Cron, Stuart Abramson, Dorothy Lewis, Claudia A. Kozinetz, William T. Shearer

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Background: Little is known about the cytokine production by peripheral blood cells of pediatric patients who have suppressed HIV-1 replication after highly active antiretroviral therapy (HAART). Objective: We sought to determine the effect of HAART on the production of TH1 and TH2 cytokines by HIV-infected children who have suppressed HIV replication. Methods: At 3- to 6-month intervals over a 5-year period, CD4+ T cells were enumerated, plasma HIV-1 RNA was measured, and levels of cytokine production by whole blood cultures were determined in 21 HIV-1-infected children. Results: Ten patients achieved an HIV-1 RNA level of less than 3000 copies/mL of plasma and maintained that level for at least 15 months (virus-suppressed [VS] group). Eleven patients had a mean HIV-1 RNA level of greater than 104 copies/mL of plasma and a mean CD4+ T-cell count of less than 500/μL of blood (active infection group). The median levels of anti-CD3-induced IL-2, IFN-γ, and IL-10 in the active infection group were significantly lower than those in the VS group after suppression of the virus. The median slope of IFN-γ production by means of PHA-stimulated culture after achieving VS status (4.04) was significantly higher than the level before VS status (-1.31, P =.004). The difference in the median slopes for IL-10 production by anti-CD3-stimulated cultures before (0.21) and after (-0.16) achieving VS status was statistically different (P =.027). Conclusion: The immune restoration of HIV-1-infected children receiving HAART might be related to an increase in IFN-γ production and a decrease in the rate of IL-10 production after virus suppression.

Original languageEnglish (US)
Pages (from-to)255-261
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume110
Issue number2
DOIs
StatePublished - Aug 2002

Keywords

  • Cytokine production
  • Highly active antiretroviral therapy
  • Immune restoration
  • Pediatric HIV
  • Virus suppression

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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