LXRβ deficient mice have reduced hepatic insulin clearance during hyperinsulinemic euglucemic clamp

Sverre Holm; Peter J. Voshol; Jan Åke Gustafsson; Hilde I. Nebb

doi:10.1016/j.bbrc.2010.01.042

LXRβ deficient mice have reduced hepatic insulin clearance during hyperinsulinemic euglucemic clamp

Sverre Holm, Peter J. Voshol, Jan Åke Gustafsson, Hilde I. Nebb

Research Institute

Research output: Contribution to journal › Article › peer-review

Abstract

The present study addresses the insulin sensitivity in mice deficient in LXRβ (LXRβ^-/-) as well as in wild type (wt) mice assessed by hyperinsulinemic euglycemic clamp. Wt and LXRβ^-/- mice were fed either a normal chow diet or a high fat and high cholesterol diet (HFCD), and insulin sensitivity was assessed by hyperinsulinemic euglycemic clamps. We show that LXRβ^-/- mice have reduced insulin clearance during hyperinsulinemic clamps upon feeding both HFCD and a regular chow diet. Moreover we also observed reduced hepatic inflammation in LXRβ^-/- mice compared to wt mice upon feeding an HFCD, despite equal levels of hepatic steatosis. In summary, our results indicate that LXRβ^-/- mice have reduced insulin clearance during hyperinsulinemic euglycemic clamps and also reduced hepatic inflammation upon feeding an HFCD for 26 weeks.

Original language	English (US)
Pages (from-to)	436-441
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	392
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2010.01.042
State	Published - Feb 12 2010

Keywords

Glucose
Hepatic steatosis
Insulin
Insulin clearance
LXR

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Molecular Biology

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10.1016/j.bbrc.2010.01.042

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title = "LXRβ deficient mice have reduced hepatic insulin clearance during hyperinsulinemic euglucemic clamp",

abstract = "The present study addresses the insulin sensitivity in mice deficient in LXRβ (LXRβ-/-) as well as in wild type (wt) mice assessed by hyperinsulinemic euglycemic clamp. Wt and LXRβ-/- mice were fed either a normal chow diet or a high fat and high cholesterol diet (HFCD), and insulin sensitivity was assessed by hyperinsulinemic euglycemic clamps. We show that LXRβ-/- mice have reduced insulin clearance during hyperinsulinemic clamps upon feeding both HFCD and a regular chow diet. Moreover we also observed reduced hepatic inflammation in LXRβ-/- mice compared to wt mice upon feeding an HFCD, despite equal levels of hepatic steatosis. In summary, our results indicate that LXRβ-/- mice have reduced insulin clearance during hyperinsulinemic euglycemic clamps and also reduced hepatic inflammation upon feeding an HFCD for 26 weeks.",

keywords = "Glucose, Hepatic steatosis, Insulin, Insulin clearance, LXR",

author = "Sverre Holm and Voshol, {Peter J.} and Gustafsson, {Jan {\AA}ke} and Nebb, {Hilde I.}",

note = "Funding Information: The authors are grateful to Christian Bindesb{\o}ll for technical assistance. This work was funded by Grants from the University of Oslo, Medical Faculty, Norwegian Research Council, the Johan Throne Holst Foundation, the Novo Nordic Foundation, EU-granted project 6th framework, CRESCENDO Grant 018652 , AstraZeneca R&D, M{\"o}lndal, Sweden, Nordic Academy for advanced study (NORDForsk), KaroBio AB, the Swedish Science Council and Netherlands Organization for Scientific Research, ZON-MW VIDI Grant 917.76.301 and Dutch Diabetes Research Career Development Grant 2005.01.003 .",

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AU - Holm, Sverre

AU - Voshol, Peter J.

AU - Gustafsson, Jan Åke

AU - Nebb, Hilde I.

N1 - Funding Information: The authors are grateful to Christian Bindesbøll for technical assistance. This work was funded by Grants from the University of Oslo, Medical Faculty, Norwegian Research Council, the Johan Throne Holst Foundation, the Novo Nordic Foundation, EU-granted project 6th framework, CRESCENDO Grant 018652 , AstraZeneca R&D, Mölndal, Sweden, Nordic Academy for advanced study (NORDForsk), KaroBio AB, the Swedish Science Council and Netherlands Organization for Scientific Research, ZON-MW VIDI Grant 917.76.301 and Dutch Diabetes Research Career Development Grant 2005.01.003 .

PY - 2010/2/12

Y1 - 2010/2/12

N2 - The present study addresses the insulin sensitivity in mice deficient in LXRβ (LXRβ-/-) as well as in wild type (wt) mice assessed by hyperinsulinemic euglycemic clamp. Wt and LXRβ-/- mice were fed either a normal chow diet or a high fat and high cholesterol diet (HFCD), and insulin sensitivity was assessed by hyperinsulinemic euglycemic clamps. We show that LXRβ-/- mice have reduced insulin clearance during hyperinsulinemic clamps upon feeding both HFCD and a regular chow diet. Moreover we also observed reduced hepatic inflammation in LXRβ-/- mice compared to wt mice upon feeding an HFCD, despite equal levels of hepatic steatosis. In summary, our results indicate that LXRβ-/- mice have reduced insulin clearance during hyperinsulinemic euglycemic clamps and also reduced hepatic inflammation upon feeding an HFCD for 26 weeks.

AB - The present study addresses the insulin sensitivity in mice deficient in LXRβ (LXRβ-/-) as well as in wild type (wt) mice assessed by hyperinsulinemic euglycemic clamp. Wt and LXRβ-/- mice were fed either a normal chow diet or a high fat and high cholesterol diet (HFCD), and insulin sensitivity was assessed by hyperinsulinemic euglycemic clamps. We show that LXRβ-/- mice have reduced insulin clearance during hyperinsulinemic clamps upon feeding both HFCD and a regular chow diet. Moreover we also observed reduced hepatic inflammation in LXRβ-/- mice compared to wt mice upon feeding an HFCD, despite equal levels of hepatic steatosis. In summary, our results indicate that LXRβ-/- mice have reduced insulin clearance during hyperinsulinemic euglycemic clamps and also reduced hepatic inflammation upon feeding an HFCD for 26 weeks.

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LXRβ deficient mice have reduced hepatic insulin clearance during hyperinsulinemic euglucemic clamp

Abstract

Keywords

ASJC Scopus subject areas

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