Long-term exposure of bacterial cells to simulated microgravity

Fathi Karouia, Madhan R. Tirumalai, Mayra A. Nelman-Gonzalez, Clarence F. Sams, Mark C. Ott, Richard C. Willson, Duane L. Pierson, George E. Fox

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

Previous space flight experience has demonstrated that microorganisms are just as ubiquitous in space habitats as they are on Earth. Numerous incidences of biofilm formation within space habitats have been reported; some of which were identified only after damage to spacecraft structures and irritation to astronaut's skin occurred. As we increase the duration of spaceflight missions, it becomes legitimate to question the long-term effects of microgravity on bacteria. To begin this assessment, Escherichia coli K-12 strain MG1655 was grown for one thousand generations (1000G) under low shear modeled microgravity. Subsequently, growth kinetics and the presence of biofilm were assessed in the 1000G strain as compared to a strain (1G) briefly exposed to LSMMG. Overall, the analysis revealed that (i) there was no obvious difference in growth kinetics between the 1G and 1000G strains, and (ii) although biofilm formation was not seen in the 1G strain it did in fact occur as exposure time increased. The results suggest that long-term exposure to the space environment likely favors biofilm formation in many organisms.

Original languageEnglish (US)
Title of host publicationInstruments, Methods, and Missions for Astrobiology XV
Volume8521
DOIs
StatePublished - Dec 1 2012
EventInstruments, Methods, and Missions for Astrobiology XV - San Diego, CA, United States
Duration: Aug 14 2012Aug 15 2012

Other

OtherInstruments, Methods, and Missions for Astrobiology XV
CountryUnited States
CitySan Diego, CA
Period8/14/128/15/12

Keywords

  • Bacterial adaptation
  • HARV
  • Low shear modeled microgravity
  • Microgravity
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

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