Long noncoding RNAs are substrates for cytoplasmic capping enzyme

Avik Mukherjee, Safirul Islam, Rachel E. Kieser, Daniel L. Kiss, Chandrama Mukherjee

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Cytoplasmic capping returns a cap to specific mRNAs, thus protecting uncapped RNAs from decay. Prior to the identification of cytoplasmic capping, uncapped mRNAs were thought to be degraded. Here, we test whether long noncoding RNAs (lncRNAs) are substrates of the cytoplasmic capping enzyme (cCE). The subcellular localisation of 14 lncRNAs associated with sarcomas were examined in U2OS osteosarcoma cells. We used 5′ rapid amplification of cDNA ends (RACE) to assay uncapped forms of these lncRNAs. Inhibiting cytoplasmic capping elevated uncapped forms of selected lncRNAs indicating a plausible role of cCE in targeting them. Analysis of published cap analysis of gene expression (CAGE) data shows increased prevalence of certain 5’-RACE cloned sequences, suggesting that these uncapped lncRNAs are targets of cytoplasmic capping.

Original languageEnglish (US)
Pages (from-to)947-961
Number of pages15
JournalFEBS Letters
Volume597
Issue number7
DOIs
StatePublished - Apr 2023

Keywords

  • 5′-RACE
  • CAGE
  • cytoplasmic capping
  • lncRNA
  • uncapped RNA ends
  • RNA, Long Noncoding/genetics
  • RNA Caps/genetics
  • Cytoplasm/metabolism
  • Cytosol/metabolism
  • RNA, Messenger/genetics

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Cell Biology

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