Abstract
Through its interaction with the low density lipoprotein (LDL) receptor, apolipoprotein (apo) B-100 is a major determinant of LDL metabolism and plasma cholesterol. Its receptor binding ability is conformation-dependent and requires its expression on the right lipoprotein particles. The structural signal that targets apoB-100 to LDL is unknown. We have microinjected a human apoB-100 minigene construct comprising <25% of the apoB-100 sequence driven by the natural apoB promoter to produce transgenic mice. The transgene product was expressed at a high level and was present exclusively in the LDL of these animals. Analysis of the responsible sequence (residues 2878-3925 of apoB-100) reveals unique structural features that may be important in its role as an LDL-targeting domain.
Original language | English (US) |
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Pages (from-to) | 20893-20898 |
Number of pages | 6 |
Journal | Journal of Biological Chemistry |
Volume | 266 |
Issue number | 31 |
State | Published - Nov 5 1991 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology