TY - JOUR
T1 - Localization of the glucocorticoid receptor in testis and accessory sexual organs of male rat
AU - Schultz, Rüdiger
AU - Isola, Jorma
AU - Parvinen, Martti
AU - Honkaniemi, Jari
AU - Wikström, Ann Charlotte
AU - Gustafsson, Jan Åke
AU - Pelto-Huikko, Markku
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1993/9
Y1 - 1993/9
N2 - Localization of glucocorticoid receptor-like immunoreactivity (GR-LI) was studied in adult rat testis, epididymis, ejaculatory duct, seminal vesicle and prostate by light and electron microscopic immunocytochemistry. In the interstitium of the testis GR-LI was seen in the nuclei of Leydig cells, macrophages, fibroblasts, smooth muscle cells and endothelial cells of blood vessels. Furthermore, GR-LI was observed in zygotene and early pachytene primary spermatocytes of some seminiferous tubules during stages XIII-XIV and I-III of the spermatogenic cycle. Other spermatogenic cells and Sertoli cells were devoid of staining. GR-LI was also found in peritubular myoid cells, fibroblasts and basal cells of the epididymis, vas deferens and prostate. Localization of GR-LI in primary spermatocytes and Leydig cells suggests that glucocorticoids directly affect spermato- and Steroidogenesis in the testis. The absence of GR-LI from functional, stromal cells of the male accessory sexual organs suggests that they are not targets for glucocorticoid hormones.
AB - Localization of glucocorticoid receptor-like immunoreactivity (GR-LI) was studied in adult rat testis, epididymis, ejaculatory duct, seminal vesicle and prostate by light and electron microscopic immunocytochemistry. In the interstitium of the testis GR-LI was seen in the nuclei of Leydig cells, macrophages, fibroblasts, smooth muscle cells and endothelial cells of blood vessels. Furthermore, GR-LI was observed in zygotene and early pachytene primary spermatocytes of some seminiferous tubules during stages XIII-XIV and I-III of the spermatogenic cycle. Other spermatogenic cells and Sertoli cells were devoid of staining. GR-LI was also found in peritubular myoid cells, fibroblasts and basal cells of the epididymis, vas deferens and prostate. Localization of GR-LI in primary spermatocytes and Leydig cells suggests that glucocorticoids directly affect spermato- and Steroidogenesis in the testis. The absence of GR-LI from functional, stromal cells of the male accessory sexual organs suggests that they are not targets for glucocorticoid hormones.
KW - Epididymis
KW - Immunohistochemistry
KW - Leydig cell
KW - Sertoli cell
KW - Spermatocyte
KW - Spermatogenesis
KW - Steroidogenesis
UR - http://www.scopus.com/inward/record.url?scp=0027323152&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027323152&partnerID=8YFLogxK
U2 - 10.1016/0303-7207(93)90036-J
DO - 10.1016/0303-7207(93)90036-J
M3 - Article
C2 - 8243801
AN - SCOPUS:0027323152
SN - 0303-7207
VL - 95
SP - 115
EP - 120
JO - Molecular and cellular endocrinology
JF - Molecular and cellular endocrinology
IS - 1-2
ER -