Localization and ontogeny of cells expressing preprodynorphin mRNA in the rat cerebral cortex

Makoto Sato, Yasuhiro Morita, Takanori Saika, Masahiro Fujita, Kazuhiro Ohhata, Masaya Tohyama

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The regional distribution of preprodynorphin (PPD) mRNA-containing cells (PPD cells) in the rat cerebral cortex was investigated by in situ hybridization histochemistry using a synthetic oligonucleotide probe. In the isocortex, PPD cells were small or medium-sized and were mainly located in layer V. While they were less numerous in the allocortex than in the isocortex. Only a few labeled cells were seen in the piriform and entorhinal cortices. In the hippocampal formation, labeled cells were observed in the granular layer of the dentate gyrus. An ontogenetic study revealed that PPD mRNA-containing cells appeared on postnatal day 7 in the isocortex and the allocortex and on day 14 in the dentate gyrus. Thereafter, they increased in number and signal intensity to reach a plateau on postnatal day 14 in both the isocortex and the allocortex and on day 35 in the dentate gyrus. The time-course of development of PPD mRNA-containing neurons in the cerebral cortex suggested that PPD-derived peptide has a neuromodulator and/or neurotransmitter role in these regions of the brain.

Original languageEnglish (US)
Pages (from-to)41-49
Number of pages9
JournalBrain Research
Volume541
Issue number1
DOIs
StatePublished - Feb 8 1991

Keywords

  • Cerebral cortex
  • Dentate gyrus
  • Dynorphin
  • Hippocampus
  • In situ hybridization histochemistry
  • Interneuron
  • Ontogeny
  • Opioid
  • Opioid receptor
  • Preprodynorphin
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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