Live-cell fluorescent microscopy platforms for real-time monitoring of polyplex-cell interaction: Basic guidelines

Ladan Parhamifar, Lin Ping Wu, Helene Andersen, S. Moein Moghimi

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A myriad of cationic polymeric delivery vehicles are currently being developed with the aim of transporting various forms of nucleic acids to mammalian cells. The complexes between polycations and nucleic acids are referred to as polyplexes. The screening for successful polyplex candidates requires interdisciplinary research platforms and techniques for a more profound understanding of biophysical properties of delivery vehicles and their biological performance, including stability, transfection efficacy and possible cytotoxicity. Fluorescent microscopy has proven to be a useful tool for real-time monitoring of performance and intracellular trafficking of polyplexes as well as for assessing cell functionality. This review highlights the application of some of the most promising fluorescent microscopy platforms in relation to polyplex-mediated transfection processes.

Original languageEnglish (US)
Pages (from-to)300-307
Number of pages8
JournalMethods
Volume68
Issue number2
DOIs
StatePublished - Jul 1 2014

Keywords

  • Fluorescent microscopy
  • Gene therapy
  • Live cell imaging
  • Nanoparticles
  • Trafficking

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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