Abstract
Liquid chromatography-mass spectrometry (LC-MS) with a nebuliser and a moving belt was used to characterise 2-nitrofluorene, its metabolites and related compounds. In total, 13 different compounds were analysed. The LC-MS interface did not affect the chromatographic performance achieved by high performance liquid chromatography (HPLC). The method allowed determination of the positions of the hydroxyl groups in different hydroxylated 2-acetylaminofluorene derivatives. The LC-MS procedure generated acceptable mass spectra with as small amounts of substance as 65 ng. The dose-response curves from the HPLC-detector and the ion-source in the LC-MS were parallel and linear.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 681-688 |
| Number of pages | 8 |
| Journal | Biological Mass Spectrometry |
| Volume | 13 |
| Issue number | 12 |
| DOIs | |
| State | Published - Jan 1 1986 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine