Liquid chromatographic‐mass spectrometric analysis of 2‐nitrofluorene and its derivatives

L. Möller, Jan-Ake Gustafsson

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Liquid chromatography-mass spectrometry (LC-MS) with a nebuliser and a moving belt was used to characterise 2-nitrofluorene, its metabolites and related compounds. In total, 13 different compounds were analysed. The LC-MS interface did not affect the chromatographic performance achieved by high performance liquid chromatography (HPLC). The method allowed determination of the positions of the hydroxyl groups in different hydroxylated 2-acetylaminofluorene derivatives. The LC-MS procedure generated acceptable mass spectra with as small amounts of substance as 65 ng. The dose-response curves from the HPLC-detector and the ion-source in the LC-MS were parallel and linear.

Original languageEnglish (US)
Pages (from-to)681-688
Number of pages8
JournalBiological Mass Spectrometry
Volume13
Issue number12
DOIs
StatePublished - Jan 1 1986

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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