Liquid chromatography-mass spectrometry (LC-MS) with a nebuliser and a moving belt was used to characterise 2-nitrofluorene, its metabolites and related compounds. In total, 13 different compounds were analysed. The LC-MS interface did not affect the chromatographic performance achieved by high performance liquid chromatography (HPLC). The method allowed determination of the positions of the hydroxyl groups in different hydroxylated 2-acetylaminofluorene derivatives. The LC-MS procedure generated acceptable mass spectra with as small amounts of substance as 65 ng. The dose-response curves from the HPLC-detector and the ion-source in the LC-MS were parallel and linear.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biological Mass Spectrometry|
|State||Published - Jan 1 1986|
ASJC Scopus subject areas
- Molecular Medicine