Liposome-mediated NGF gene transfection increases ChAT activity in CNS cell cultures

Weidong Le, Keyi Yang, Janet S. Whitson, Guy L. Clifton, Ronald L. Hayes

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Liposome-mediated NGF gene transfection has been shown to increase the expression and secretion of NGF in primary rat septo-hippocampal cell cultures. Here we report that along with increased NGF expression, the activity of choline acetyltransferase, the synthetic enzyme for acetylcholine, is increased by 18% within 2 days, by 41% within 4 days and by 32% within 8 days after NGF gene transfection in septo-hippocampal cell cultures. This result further confirms that biologically active NGF is functionally expressed in septo-hippocampal cells when transfected with cDNA for NGF completed with liposomes.

Original languageEnglish (US)
Pages (from-to)710-712
Number of pages3
JournalNeuroReport
Volume7
Issue number3
DOIs
StatePublished - 1996

Keywords

  • ChAT
  • Gene therapy
  • Liposome
  • NGF
  • Septo-hippocampal cell culture

ASJC Scopus subject areas

  • Neuroscience(all)

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