TY - JOUR
T1 - Lipoprotein(a)
T2 - Structure, Metabolism and Epidemiology
AU - Morrisett, Joel D.
AU - Guyton, John R.
AU - Gaubatz, John W.
AU - Gotto, Antonio
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1987/1
Y1 - 1987/1
N2 - This chapter discusses structure, metabolism, and epidemiology of lipoprotein(a) [Lp(a)]. Lp(a) has been purified by gel filtration chromatography on Bio-Gel A-15m and has an apparent diameter of 262 Å as determined by quasielastic light scattering. Upon extended storage under low-salt conditions, the lipoprotein undergoes self-association to a particle with an apparent diameter of about 420 Å. The molecular weight of unfractionated Lp(a) has been measured by several different techniques including gel filtration chromatography, sedimentation equilibrium, and quasielastic light scattering. Lp(a) exhibits slow pre-β mobility when electrophoresed in agarose. One of the distinguishing physical properties of Lp(a) is its high tendency toward aggregation, especially at high concentrations (> 5–10 mg/ml). Several different assays have been developed for quantitating Lp(a) in fresh and frozen sera. Each of these assays is based upon an immunochemical reaction between the apo(a) antigen and an antibody directed against one or more of its determinants.
AB - This chapter discusses structure, metabolism, and epidemiology of lipoprotein(a) [Lp(a)]. Lp(a) has been purified by gel filtration chromatography on Bio-Gel A-15m and has an apparent diameter of 262 Å as determined by quasielastic light scattering. Upon extended storage under low-salt conditions, the lipoprotein undergoes self-association to a particle with an apparent diameter of about 420 Å. The molecular weight of unfractionated Lp(a) has been measured by several different techniques including gel filtration chromatography, sedimentation equilibrium, and quasielastic light scattering. Lp(a) exhibits slow pre-β mobility when electrophoresed in agarose. One of the distinguishing physical properties of Lp(a) is its high tendency toward aggregation, especially at high concentrations (> 5–10 mg/ml). Several different assays have been developed for quantitating Lp(a) in fresh and frozen sera. Each of these assays is based upon an immunochemical reaction between the apo(a) antigen and an antibody directed against one or more of its determinants.
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U2 - 10.1016/S0167-7306(08)60198-2
DO - 10.1016/S0167-7306(08)60198-2
M3 - Article
AN - SCOPUS:77956800874
SN - 0167-7306
VL - 14
SP - 129
EP - 152
JO - New Comprehensive Biochemistry
JF - New Comprehensive Biochemistry
IS - C
ER -