Lipoprotein-apoprotein exchange in aqueous systems: Relevance to the occurrence of APOA-I and APOC proteins in a common particle

Henry J. Pownall, Quein Pao, Michael Rohde, Antonio Gotto

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Human plasma high density lipoproteins (HDL) were labeled in vitro with [125I]apoA-I. Chromatography of the [125I]HDL on Sephacryl S-200 revealed that a certain fraction of [125I]apoA-I readily dissociates from the intact particle over a wide range of HDL concentrations. The relatively constant value of the dissociated apoA-I concentration observed at various HDL concentrations suggests that a "critical monomer concentration" of apoA-I is in equilibrium with the parent lipoprotein. Addition of [131I]apoC proteins to HDL induces additional dissociation of oligomeric apoA-I with the concomitant incorporation of apoC into a new particle of about 460,000 daltons.

Original languageEnglish (US)
Pages (from-to)408-414
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume85
Issue number1
DOIs
StatePublished - Nov 14 1978

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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