TY - JOUR
T1 - LincRNA-p21 Suppresses Target mRNA Translation
AU - Yoon, Je Hyun
AU - Abdelmohsen, Kotb
AU - Srikantan, Subramanya
AU - Yang, Xiaoling
AU - Martindale, Jennifer L.
AU - De, Supriyo
AU - Huarte, Maite
AU - Zhan, Ming
AU - Becker, Kevin G.
AU - Gorospe, Myriam
PY - 2012/8/24
Y1 - 2012/8/24
N2 - Mammalian long intergenic noncoding RNAs (lincRNAs) are best known for modulating transcription. Here we report a posttranscriptional function for lincRNA-p21 as a modulator of translation. Association of the RNA-binding protein HuR with lincRNA-p21 favored the recruitment of let-7/Ago2 to lincRNA-p21, leading to lower lincRNA-p21 stability. Under reduced HuR levels, lincRNA-p21 accumulated in human cervical carcinoma HeLa cells, increasing its association with JUNB and CTNNB1 mRNAs and selectively lowering their translation. With elevated HuR, lincRNA-p21 levels declined, which in turn derepressed JunB and β-catenin translation and increased the levels of these proteins. We propose that HuR controls translation of a subset of target mRNAs by influencing lincRNA-p21 levels. Our findings uncover a role for lincRNA as a posttranscriptional inhibitor of translation.
AB - Mammalian long intergenic noncoding RNAs (lincRNAs) are best known for modulating transcription. Here we report a posttranscriptional function for lincRNA-p21 as a modulator of translation. Association of the RNA-binding protein HuR with lincRNA-p21 favored the recruitment of let-7/Ago2 to lincRNA-p21, leading to lower lincRNA-p21 stability. Under reduced HuR levels, lincRNA-p21 accumulated in human cervical carcinoma HeLa cells, increasing its association with JUNB and CTNNB1 mRNAs and selectively lowering their translation. With elevated HuR, lincRNA-p21 levels declined, which in turn derepressed JunB and β-catenin translation and increased the levels of these proteins. We propose that HuR controls translation of a subset of target mRNAs by influencing lincRNA-p21 levels. Our findings uncover a role for lincRNA as a posttranscriptional inhibitor of translation.
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U2 - 10.1016/j.molcel.2012.06.027
DO - 10.1016/j.molcel.2012.06.027
M3 - Article
C2 - 22841487
AN - SCOPUS:84865379361
VL - 47
SP - 648
EP - 655
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 4
ER -