TY - JOUR
T1 - Life-long in vivo cell-lineage tracing shows that no oogenesis originates from putative germline stem cells in adult mice
AU - Zhang, Hua
AU - Liu, Lian
AU - Li, Xin
AU - Busayavalasa, Kiran
AU - Shen, Yan
AU - Hovatta, Outi
AU - Gustafsson, Jan Åke
AU - Liu, Kui
PY - 2014/12/16
Y1 - 2014/12/16
N2 - Whether or not oocyte regeneration occurs in adult life has been the subject of much debate. In this study, we have traced germcell lineages over the life spans of three genetically modified mouse models and provide direct evidence that oogenesis does not originate from any germline stem cells (GSCs) in adult mice. By selective ablation of all existing oocytes in a Gdf9-Cre;iDTR mouse model, we have demonstrated that no new germ cells were ever regenerated under pathological conditions. By in vivo tracing of oocytes and follicles in the Sohlh1-CreERT2;R26R and Foxl2-CreERT2;mT/mG mouse models, respectively, we have shown that the initial pool of oocytes is the only source of germ cells throughout the life span of the mice and that no adult oogenesis ever occurs under physiological conditions. Our findings clearly show that there are no GSCs that contribute to adult oogenesis in mice and that the initial pool of oocytes formed in early life is the only source of germ cells throughout the entire reproductive life span.
AB - Whether or not oocyte regeneration occurs in adult life has been the subject of much debate. In this study, we have traced germcell lineages over the life spans of three genetically modified mouse models and provide direct evidence that oogenesis does not originate from any germline stem cells (GSCs) in adult mice. By selective ablation of all existing oocytes in a Gdf9-Cre;iDTR mouse model, we have demonstrated that no new germ cells were ever regenerated under pathological conditions. By in vivo tracing of oocytes and follicles in the Sohlh1-CreERT2;R26R and Foxl2-CreERT2;mT/mG mouse models, respectively, we have shown that the initial pool of oocytes is the only source of germ cells throughout the life span of the mice and that no adult oogenesis ever occurs under physiological conditions. Our findings clearly show that there are no GSCs that contribute to adult oogenesis in mice and that the initial pool of oocytes formed in early life is the only source of germ cells throughout the entire reproductive life span.
KW - Follicle tracing
KW - Genetically modified mouse models
KW - Life-long observations
KW - No postnatal oocyte regeneration
KW - Oocyte tracing
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U2 - 10.1073/pnas.1421047111
DO - 10.1073/pnas.1421047111
M3 - Article
C2 - 25453063
AN - SCOPUS:84919401701
VL - 111
SP - 17983
EP - 17988
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 50
ER -