Length-based separation of short DNA using nanoslit arrays

E. A. Strychalski, H. W. Lau, L. Archer, Harold G. Craighead

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Fused silica nanofluidic devices containing either nanoslit arrays or individual nanoslits were used to investigate the electric field dependent separation of fluorescently labeled, double-stranded deoxyribonucleic acid (DNA) with lengths of 55 bp, 259 bp, and 753 bp. Initial results provided experimental evidence to suggest that the breakdown of Ogston sieving of short DNA can be supplanted at higher average electric fields values in the array, Eave, to recover length-based separation.

Original languageEnglish
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages856-858
Number of pages3
StatePublished - Jan 1 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Keywords

  • DNA
  • Electrophoresis
  • Nanofluidic
  • Nanoslit
  • Separation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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