Lactobacillus saerimneri and Lactobacillus ruminis: Novel human-derived probiotic strains with immunomodulatory activities

Malai Taweechotipatr, Chandra Iyer, Jennifer K. Spinler, James Versalovic, Somying Tumwasorn

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Human-derived lactobacilli were isolated from fecal samples of healthy volunteers. Forty-six isolates from different volunteers were selected and investigated for their immunomodulatory properties. Conditioned medium from each isolate was assessed for its effect on tumor necrosis factor (TNF) production in lipopolysaccharide-activated THP-1 monocytes. Of 46 Lactobacillus isolates, 12 significantly inhibited TNF production in varying magnitude. Lactobacillus strain TH58 displayed the most potent TNF-inhibitory activity (70% inhibition). In contrast, Lactobacillus strain TH14 exhibited immunostimulatory property by activating TNF production in THP-1 monocytes. Lactobacillus TH14 induced nuclear factor-κB (NF-κB) activation in the absence of lipopolysaccharide stimulation, whereas Lactobacillus TH58 had no effect on NF-κB signaling, irrespective of lipopolysaccharide stimulation. Strain TH58 was identified as Lactobacillus saerimneri and strain TH14 as Lactobacillus ruminis by sequence analysis of their 16S rRNA genes. This is the first report of a human isolate of L. saerimneri with TNF-inhibitory activity and L. ruminis, an indigenous species to humans, with TNF stimulatory activity. Our data suggest the potential use of these two strains as immunoprobiotic candidates.

Original languageEnglish (US)
Pages (from-to)65-72
Number of pages8
JournalFEMS Microbiology Letters
Volume293
Issue number1
DOIs
StatePublished - Apr 2009

Keywords

  • Anti-inflammatory
  • Immunoprobiotics
  • Immunostimulatory
  • Lactobacillus ruminis
  • Lactobacillus saerimneri
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

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