Lactate dehydrogenase assay for assessment of polycation cytotoxicity

Ladan Parhamifar, Helene Andersen, S. Moein Moghimi

Research output: Chapter in Book/Report/Conference proceedingChapter

28 Scopus citations

Abstract

Cellular toxicity and/or cell death entail complex mechanisms that require detailed evaluation for proper characterization. A detailed mechanistic assessment of cytotoxicity is essential for design and construction of more effective polycations for nucleic acid delivery. A single toxicity assay cannot stand alone in determining the type and extent of damage or cell death mechanism. In this chapter we describe a lactate dehydrogenase (LDH) assay for high-Throughput screening that can be used as a starting point for further detailed cytotoxicity determination. LDH release is considered an early event in necrosis but a late event in apoptosis. An accurate temporal assessment of the toxic responses is crucial as late apoptosis may convert into necrosis as well as in situations where cell death is initiated without any visible cell morphological changes or responses in assays measuring late events, resulting in early ongoing toxicity being overlooked.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages13-22
Number of pages10
Volume948
ISBN (Print)9781627031394
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume948
ISSN (Print)10643745

Keywords

  • Apoptosis
  • Lactate dehydrogenase
  • Necrosis
  • Plasma membrane damage
  • Polycations
  • Toxicity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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