Label-free proteomics and systems biology analysis of mycobacterial phagosomes in dendritic cells and macrophages

Qingbo Li, Christopher R. Singh, Shuyi Ma, Nathan D. Price, Chinnaswamy Jagannath

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Proteomics has been applied to study intracellular bacteria and phagocytic vacuoles in different host cell lines, especially macrophages (Mφs). For mycobacterial phagosomes, few studies have identified over several hundred proteins for systems assessment of the phagosome maturation and antigen presentation pathways. More importantly, there has been a scarcity in publication on proteomic characterization of mycobacterial phagosomes in dendritic cells (DCs). In this work, we report a global proteomic analysis of Mφ and DC phagosomes infected with a virulent, an attenuated, and a vaccine strain of mycobacteria. We used label-free quantitative proteomics and bioinformatics tools to decipher the regulation of phagosome maturation and antigen presentation pathways in Mφs and DCs. We found that the phagosomal antigen presentation pathways are repressed more in DCs than in Mφs. The results suggest that virulent mycobacteria might co-opt the host immune system to stimulate granuloma formation for persistence while minimizing the antimicrobial immune response to enhance mycobacterial survival. The studies on phagosomal proteomes have also shown promise in discovering new antigen presentation mechanisms that a professional antigen presentation cell might use to overcome the mycobacterial blockade of conventional antigen presentation pathways.

Original languageEnglish (US)
Pages (from-to)2425-2439
Number of pages15
JournalJournal of Proteome Research
Volume10
Issue number5
DOIs
StatePublished - May 6 2011
Externally publishedYes

Keywords

  • Mycobacterium tuberculosis
  • antigen presentation
  • dendritic cell
  • macrophage
  • phagosome
  • proteomics
  • systems biology

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Label-free proteomics and systems biology analysis of mycobacterial phagosomes in dendritic cells and macrophages'. Together they form a unique fingerprint.

Cite this