Label-free optical imaging of cell function and collagen structure for cell-based therapies

Linghao Hu; Samantha Morganti; Uyen Nguyen; Oscar R. Benavides; Alex J. Walsh

doi:10.1016/j.cobme.2022.100433

Label-free optical imaging of cell function and collagen structure for cell-based therapies

Linghao Hu, Samantha Morganti, Uyen Nguyen, Oscar R. Benavides, Alex J. Walsh

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Cell-based therapies harness functional cells or tissues to mediate healing and treat disease. Assessment of cellular therapeutics requires methods that are non-destructive to ensure therapies remain viable and uncontaminated for use in patients. Optical imaging of endogenous collagen, by second-harmonic generation, and the metabolic coenzymes NADH and FAD, by autofluorescence microscopy, provides tissue structure and cellular information. Here, we review applications of label-free nonlinear optical imaging of cellular metabolism and collagen second-harmonic generation for assessing cell-based therapies. Additionally, we discuss the potential of label-free imaging for quality control of cell-based therapies, as well as the current limitations and potential future directions of label-free imaging technologies.

Original language	English (US)
Article number	100433
Journal	Current Opinion in Biomedical Engineering
Volume	25
DOIs	https://doi.org/10.1016/j.cobme.2022.100433
State	Published - Mar 2023

Keywords

Collagen
Cytotherapy
Fluorescence
Metabolism
Microscopy
Regenerative engineering

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomaterials
Biomedical Engineering

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10.1016/j.cobme.2022.100433

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title = "Label-free optical imaging of cell function and collagen structure for cell-based therapies",

abstract = "Cell-based therapies harness functional cells or tissues to mediate healing and treat disease. Assessment of cellular therapeutics requires methods that are non-destructive to ensure therapies remain viable and uncontaminated for use in patients. Optical imaging of endogenous collagen, by second-harmonic generation, and the metabolic coenzymes NADH and FAD, by autofluorescence microscopy, provides tissue structure and cellular information. Here, we review applications of label-free nonlinear optical imaging of cellular metabolism and collagen second-harmonic generation for assessing cell-based therapies. Additionally, we discuss the potential of label-free imaging for quality control of cell-based therapies, as well as the current limitations and potential future directions of label-free imaging technologies.",

keywords = "Collagen, Cytotherapy, Fluorescence, Metabolism, Microscopy, Regenerative engineering",

author = "Linghao Hu and Samantha Morganti and Uyen Nguyen and Benavides, \{Oscar R.\} and Walsh, \{Alex J.\}",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2023",

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doi = "10.1016/j.cobme.2022.100433",

language = "English (US)",

volume = "25",

journal = "Current Opinion in Biomedical Engineering",

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T1 - Label-free optical imaging of cell function and collagen structure for cell-based therapies

AU - Hu, Linghao

AU - Morganti, Samantha

AU - Nguyen, Uyen

AU - Benavides, Oscar R.

AU - Walsh, Alex J.

PY - 2023/3

Y1 - 2023/3

N2 - Cell-based therapies harness functional cells or tissues to mediate healing and treat disease. Assessment of cellular therapeutics requires methods that are non-destructive to ensure therapies remain viable and uncontaminated for use in patients. Optical imaging of endogenous collagen, by second-harmonic generation, and the metabolic coenzymes NADH and FAD, by autofluorescence microscopy, provides tissue structure and cellular information. Here, we review applications of label-free nonlinear optical imaging of cellular metabolism and collagen second-harmonic generation for assessing cell-based therapies. Additionally, we discuss the potential of label-free imaging for quality control of cell-based therapies, as well as the current limitations and potential future directions of label-free imaging technologies.

AB - Cell-based therapies harness functional cells or tissues to mediate healing and treat disease. Assessment of cellular therapeutics requires methods that are non-destructive to ensure therapies remain viable and uncontaminated for use in patients. Optical imaging of endogenous collagen, by second-harmonic generation, and the metabolic coenzymes NADH and FAD, by autofluorescence microscopy, provides tissue structure and cellular information. Here, we review applications of label-free nonlinear optical imaging of cellular metabolism and collagen second-harmonic generation for assessing cell-based therapies. Additionally, we discuss the potential of label-free imaging for quality control of cell-based therapies, as well as the current limitations and potential future directions of label-free imaging technologies.

KW - Collagen

KW - Cytotherapy

KW - Fluorescence

KW - Metabolism

KW - Microscopy

KW - Regenerative engineering

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JO - Current Opinion in Biomedical Engineering

JF - Current Opinion in Biomedical Engineering

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ER -

Label-free optical imaging of cell function and collagen structure for cell-based therapies

Abstract

Keywords

ASJC Scopus subject areas

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